1rxe

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[[Image:1rxe.gif|left|200px]]
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{{STRUCTURE_1rxe| PDB=1rxe | SCENE= }}
{{STRUCTURE_1rxe| PDB=1rxe | SCENE= }}
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'''ArsC complexed with MNB'''
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===ArsC complexed with MNB===
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==Overview==
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Structural insights into formation of the complex between the ubiquitous thiol-disulfide oxidoreductase thioredoxin and its oxidized substrate are under-documented owing to its entropical instability. In vitro, it is possible via a reaction with 5,5'-dithiobis-(2-nitrobenzoic acid) to make a stable mixed-disulfide complex between thioredoxin from Staphylococcus aureus and one of its substrates, oxidized pI258 arsenate reductase (ArsC) from S. aureus. In the absence of the crystal structure of an ArsC-thioredoxin complex, the structures of two precursors of the complex, the ArsC triple mutant ArsC C10SC15AC82S and its 5-thio-2-nitrobenzoic acid (TNB) adduct, were determined. The ArsC triple mutant has a structure very similar to that of the reduced form of wild-type ArsC, with a folded redox helix and a buried catalytic Cys89. In the adduct form, the TNB molecule is buried in a hydrophobic pocket and the disulfide bridge between TNB and Cys89 is sterically inaccessible to thioredoxin. In order to form a mixed disulfide between ArsC and thioredoxin, a change in the orientation of the TNB-Cys89 disulfide in the structure is necessary.
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The line below this paragraph, {{ABSTRACT_PUBMED_15159594}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15159594 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15159594}}
==About this Structure==
==About this Structure==
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[[Category: Mnb]]
[[Category: Mnb]]
[[Category: Tnb]]
[[Category: Tnb]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:01:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:06:02 2008''

Revision as of 12:06, 27 July 2008

Image:1rxe.png

Template:STRUCTURE 1rxe

ArsC complexed with MNB

Template:ABSTRACT PUBMED 15159594

About this Structure

1RXE is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

The structure of a triple mutant of pI258 arsenate reductase from Staphylococcus aureus and its 5-thio-2-nitrobenzoic acid adduct., Messens J, Van Molle I, Vanhaesebrouck P, Van Belle K, Wahni K, Martins JC, Wyns L, Loris R, Acta Crystallogr D Biol Crystallogr. 2004 Jun;60(Pt 6):1180-4. Epub 2004, May 21. PMID:15159594

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