This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1u78

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1u78.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1u78.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1u78| PDB=1u78 | SCENE= }}
{{STRUCTURE_1u78| PDB=1u78 | SCENE= }}
-
'''Structure of the bipartite DNA-binding domain of Tc3 transposase bound to transposon DNA'''
+
===Structure of the bipartite DNA-binding domain of Tc3 transposase bound to transposon DNA===
-
==Overview==
+
<!--
-
The bipartite DNA-binding domain of Tc3 transposase, Tc3A, was crystallized in complex with its transposon recognition sequence. In the structure the two DNA-binding domains form structurally related helix-turn-helix (HTH) motifs. They both bind to the major groove on a single DNA oligomer, separated by a linker that interacts closely with the minor groove. The structure resembles that of the transcription factor Pax6 DNA-binding domain, but the relative orientation of the HTH-domain is different. The DNA conformation is distorted, characterized by local narrowing of the minor groove and bends at both ends. The protein-DNA recognition takes place through base and backbone contacts, as well as shape-recognition of the distortions in the DNA. Charged interactions are primarily found in the N-terminal domain and the linker indicating that these may form the initial contact area. Two independent dimer interfaces could be relevant for bringing together transposon ends and for binding to a direct repeat site in the transposon end. In contrast to the Tn5 synaptic complex, the two Tc3A DNA-binding domains bind to a single Tc3 transposon end.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15304566}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15304566 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15304566}}
==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Transposase]]
[[Category: Transposase]]
[[Category: Transposon dna]]
[[Category: Transposon dna]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:50:29 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:08:58 2008''

Revision as of 12:08, 27 July 2008

Image:1u78.png

Template:STRUCTURE 1u78

Structure of the bipartite DNA-binding domain of Tc3 transposase bound to transposon DNA

Template:ABSTRACT PUBMED 15304566

About this Structure

1U78 is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.

Reference

Structural analysis of the bipartite DNA-binding domain of Tc3 transposase bound to transposon DNA., Watkins S, van Pouderoyen G, Sixma TK, Nucleic Acids Res. 2004 Aug 10;32(14):4306-12. Print 2004. PMID:15304566

Page seeded by OCA on Sun Jul 27 15:08:58 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1u78"
Personal tools