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1tgg

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[[Image:1tgg.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1tgg| PDB=1tgg | SCENE= }}
{{STRUCTURE_1tgg| PDB=1tgg | SCENE= }}
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'''RH3 DESIGNED RIGHT-HANDED COILED COIL TRIMER'''
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===RH3 DESIGNED RIGHT-HANDED COILED COIL TRIMER===
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==Overview==
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Designing new protein folds requires a method for simultaneously optimizing the conformation of the backbone and the side-chains. One approach to this problem is the use of a parameterized backbone, which allows the systematic exploration of families of structures. We report the crystal structure of RH3, a right-handed, three-helix coiled coil that was designed using a parameterized backbone and detailed modeling of core packing. This crystal structure was determined using another rationally designed feature, a metal-binding site that permitted experimental phasing of the X-ray data. RH3 adopted the intended fold, which has not been observed previously in biological proteins. Unanticipated structural asymmetry in the trimer was a principal source of variation within the RH3 structure. The sequence of RH3 differs from that of a previously characterized right-handed tetramer, RH4, at only one position in each 11 amino acid sequence repeat. This close similarity indicates that the design method is sensitive to the core packing interactions that specify the protein structure. Comparison of the structures of RH3 and RH4 indicates that both steric overlap and cavity formation provide strong driving forces for oligomer specificity.
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The line below this paragraph, {{ABSTRACT_PUBMED_15313624}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15313624 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15313624}}
==About this Structure==
==About this Structure==
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[[Category: Coiled coil]]
[[Category: Coiled coil]]
[[Category: De novo design]]
[[Category: De novo design]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:55:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:10:27 2008''

Revision as of 12:10, 27 July 2008

Image:1tgg.png

Template:STRUCTURE 1tgg

RH3 DESIGNED RIGHT-HANDED COILED COIL TRIMER

Template:ABSTRACT PUBMED 15313624

About this Structure

Full crystallographic information is available from OCA.

Reference

Structural test of the parameterized-backbone method for protein design., Plecs JJ, Harbury PB, Kim PS, Alber T, J Mol Biol. 2004 Sep 3;342(1):289-97. PMID:15313624

Page seeded by OCA on Sun Jul 27 15:10:27 2008

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