1nx9

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[[Image:1nx9.gif|left|200px]]
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{{Seed}}
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[[Image:1nx9.png|left|200px]]
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{{STRUCTURE_1nx9| PDB=1nx9 | SCENE= }}
{{STRUCTURE_1nx9| PDB=1nx9 | SCENE= }}
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'''Acetobacter turbidans alpha-amino acid ester hydrolase S205A mutant complexed with ampicillin'''
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===Acetobacter turbidans alpha-amino acid ester hydrolase S205A mutant complexed with ampicillin===
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==Overview==
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The alpha-amino acid ester hydrolase (AEH) from Acetobacter turbidans is a bacterial enzyme catalyzing the hydrolysis and synthesis of beta-lactam antibiotics. The crystal structures of the native enzyme, both unliganded and in complex with the hydrolysis product D-phenylglycine are reported, as well as the structures of an inactive mutant (S205A) complexed with the substrate ampicillin, and an active site mutant (Y206A) with an increased tendency to catalyze antibiotic production rather than hydrolysis. The structure of the native enzyme shows an acyl binding pocket, in which D-phenylglycine binds, and an additional space that is large enough to accommodate the beta-lactam moiety of an antibiotic. In the S205A mutant, ampicillin binds in this pocket in a non-productive manner, making extensive contacts with the side chain of Tyr(112), which also participates in oxyanion hole formation. In the Y206A mutant, the Tyr(112) side chain has moved with its hydroxyl group toward the catalytic serine. Because this changes the properties of the beta-lactam binding site, this could explain the increased beta-lactam transferase activity of this mutant.
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The line below this paragraph, {{ABSTRACT_PUBMED_16377627}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16377627 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16377627}}
==About this Structure==
==About this Structure==
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[[Category: Alpha/beta hydrolase]]
[[Category: Alpha/beta hydrolase]]
[[Category: Jellyroll]]
[[Category: Jellyroll]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:05:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:14:45 2008''

Revision as of 12:14, 27 July 2008

Image:1nx9.png

Template:STRUCTURE 1nx9

Acetobacter turbidans alpha-amino acid ester hydrolase S205A mutant complexed with ampicillin

Template:ABSTRACT PUBMED 16377627

About this Structure

1NX9 is a Single protein structure of sequence from Acetobacter pasteurianus. Full crystallographic information is available from OCA.

Reference

Acetobacter turbidans alpha-amino acid ester hydrolase: how a single mutation improves an antibiotic-producing enzyme., Barends TR, Polderman-Tijmes JJ, Jekel PA, Williams C, Wybenga G, Janssen DB, Dijkstra BW, J Biol Chem. 2006 Mar 3;281(9):5804-10. Epub 2005 Dec 23. PMID:16377627

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