1k4z

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Revision as of 16:44, 20 November 2007

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spinqualitylabelsall models 1k4z, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

C-terminal Domain of Cyclase Associated Protein

Overview

Cyclase-associated protein (CAP or Srv2p) is a modular actin monomer, binding protein that directly regulates filament dynamics and has been, implicated in a number of complex developmental and morphological, processes, including mRNA localization and the establishment of cell, polarity. The crystal structure of the C-terminal dimerization and actin, monomer binding domain (C-CAP) reveals a highly unusual dimer, composed of, monomers possessing six coils of right-handed beta-helix flanked by, antiparallel beta-strands. Domain swapping, involving the last two strands, of each monomer, results in the formation of an extended dimer with an, extensive interface. This structural and biochemical characterization, provides new insights into the organization and potential mechanistic, properties of the multiprotein assemblies that integrate dynamic actin, processes into the overall physiology of the cell. An unanticipated, finding is that the unique tertiary structure of the C-CAP monomer, provides a structural model for a wide range of molecules, including RP2, and cofactor C, proteins involved in X-linked retinitis pigmentosa and, tubulin maturation, respectively, as well as several uncharacterized, proteins that exhibit very diverse domain organizations. Thus, the unusual, right-handed beta-helical fold present in C-CAP appears to support a wide, range of biological functions.

About this Structure

1K4Z is a Single protein structure of sequence from Saccharomyces cerevisiae. This structure superseeds the now removed PDB entry 1F5I. Full crystallographic information is available from OCA.

Reference

Crystal structure of the actin binding domain of the cyclase-associated protein., Dodatko T, Fedorov AA, Grynberg M, Patskovsky Y, Rozwarski DA, Jaroszewski L, Aronoff-Spencer E, Kondraskina E, Irving T, Godzik A, Almo SC, Biochemistry. 2004 Aug 24;43(33):10628-41. PMID:15311924

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