2np9

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{{STRUCTURE_2np9| PDB=2np9 | SCENE= }}
{{STRUCTURE_2np9| PDB=2np9 | SCENE= }}
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'''Crystal structure of a dioxygenase in the Crotonase superfamily'''
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===Crystal structure of a dioxygenase in the Crotonase superfamily===
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==Overview==
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Enzyme-catalysed oxidations are some of the most common transformations in primary and secondary metabolism. The vancomycin biosynthetic enzyme DpgC belongs to a small class of oxygenation enzymes that are not dependent on an accessory cofactor or metal ion. The detailed mechanism of cofactor-independent oxygenases has not been established. Here we report the first structure of an enzyme of this oxygenase class in complex with a bound substrate mimic. The use of a designed, synthetic substrate analogue allows unique insights into the chemistry of oxygen activation. The structure confirms the absence of cofactors, and electron density consistent with molecular oxygen is present adjacent to the site of oxidation on the substrate. Molecular oxygen is bound in a small hydrophobic pocket and the substrate provides the reducing power to activate oxygen for downstream chemical steps. Our results resolve the unique and complex chemistry of DpgC, a key enzyme in the biosynthetic pathway of an important class of antibiotics. Furthermore, mechanistic parallels exist between DpgC and cofactor-dependent flavoenzymes, providing information regarding the general mechanism of enzymatic oxygen activation.
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The line below this paragraph, {{ABSTRACT_PUBMED_17507985}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17507985 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17507985}}
==About this Structure==
==About this Structure==
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[[Category: Widboom, P F.]]
[[Category: Widboom, P F.]]
[[Category: Protein inhibitor complex]]
[[Category: Protein inhibitor complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:44:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:29:31 2008''

Revision as of 12:29, 27 July 2008

Image:2np9.png

Template:STRUCTURE 2np9

Crystal structure of a dioxygenase in the Crotonase superfamily

Template:ABSTRACT PUBMED 17507985

About this Structure

2NP9 is a Single protein structure of sequence from Streptomyces toyocaensis. Full crystallographic information is available from OCA.

Reference

Structural basis for cofactor-independent dioxygenation in vancomycin biosynthesis., Widboom PF, Fielding EN, Liu Y, Bruner SD, Nature. 2007 May 17;447(7142):342-5. PMID:17507985

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