1u0w

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{{STRUCTURE_1u0w| PDB=1u0w | SCENE= }}
{{STRUCTURE_1u0w| PDB=1u0w | SCENE= }}
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'''An Aldol Switch Discovered in Stilbene Synthases Mediates Cyclization Specificity of Type III Polyketide Synthases: 18xCHS+resveratrol Structure'''
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===An Aldol Switch Discovered in Stilbene Synthases Mediates Cyclization Specificity of Type III Polyketide Synthases: 18xCHS+resveratrol Structure===
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==Overview==
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Stilbene synthase (STS) and chalcone synthase (CHS) each catalyze the formation of a tetraketide intermediate from a CoA-tethered phenylpropanoid starter and three molecules of malonyl-CoA, but use different cyclization mechanisms to produce distinct chemical scaffolds for a variety of plant natural products. Here we present the first STS crystal structure and identify, by mutagenic conversion of alfalfa CHS into a functional stilbene synthase, the structural basis for the evolution of STS cyclization specificity in type III polyketide synthase (PKS) enzymes. Additional mutagenesis and enzymatic characterization confirms that electronic effects rather than steric factors balance competing cyclization specificities in CHS and STS. Finally, we discuss the problematic in vitro reconstitution of plant stilbenecarboxylate pathways, using insights from existing biomimetic polyketide cyclization studies to generate a novel mechanistic hypothesis to explain stilbenecarboxylate biosynthesis.
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(as it appears on PubMed at http://www.pubmed.gov), where 15380179 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15380179}}
==About this Structure==
==About this Structure==
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[[Category: Thiolase fold]]
[[Category: Thiolase fold]]
[[Category: Type iii polyketide synthase]]
[[Category: Type iii polyketide synthase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:37:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:31:13 2008''

Revision as of 12:31, 27 July 2008

Image:1u0w.png

Template:STRUCTURE 1u0w

An Aldol Switch Discovered in Stilbene Synthases Mediates Cyclization Specificity of Type III Polyketide Synthases: 18xCHS+resveratrol Structure

Template:ABSTRACT PUBMED 15380179

About this Structure

1U0W is a Single protein structure of sequence from Medicago sativa. Full crystallographic information is available from OCA.

Reference

An aldol switch discovered in stilbene synthases mediates cyclization specificity of type III polyketide synthases., Austin MB, Bowman ME, Ferrer JL, Schroder J, Noel JP, Chem Biol. 2004 Sep;11(9):1179-94. PMID:15380179

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