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2yqh

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{{STRUCTURE_2yqh| PDB=2yqh | SCENE= }}
{{STRUCTURE_2yqh| PDB=2yqh | SCENE= }}
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'''Crystal structure of uridine-diphospho-N-acetylglucosamine pyrophosphorylase from Candida albicans, in the substrate-binding form'''
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===Crystal structure of uridine-diphospho-N-acetylglucosamine pyrophosphorylase from Candida albicans, in the substrate-binding form===
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==Overview==
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Uridine-diphospho-N-acetylglucosamine (UDP-GlcNAc) is a precursor of the bacterial and fungal cell wall. It is also used in a component of N-linked glycosylation and the glycosylphosphoinositol anchor of eukaryotic proteins. It is synthesized from N-acetylglucosamine-1-phosphate (GlcNAc-1-P) and uridine-5'-triphosphate (UTP) by UDP-GlcNAc pyrophosphorylase (UAP). This is an S(N)2 reaction; the non-esterified oxygen atom of the GlcNAc-1-P phosphate group attacks the alpha-phosphate group of UTP. We determined crystal structures of UAP from Candida albicans (CaUAP1) without any ligands and also complexed with its substrate or with its product. The series of structures in different forms shows the induced fit movements of CaUAP1. Three loops approaching the ligand molecule close the active site when ligand is bound. In addition, Lys-421, instead of the metal ion in prokaryotic UAPs, is coordinated by both phosphate groups of UDP-Glc-NAc and acts as a cofactor. However, a magnesium ion enhances the enzymatic activity of CaUAP1, and thus we propose that the magnesium ion increases the affinity between UTP and the enzyme by coordinating to the alpha- and gamma-phosphate group of UTP.
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(as it appears on PubMed at http://www.pubmed.gov), where 17392279 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17392279}}
==About this Structure==
==About this Structure==
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[[Category: Pyrophosphorylase]]
[[Category: Pyrophosphorylase]]
[[Category: Uridine-diphospho-n-acetylglucosamine]]
[[Category: Uridine-diphospho-n-acetylglucosamine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:36:02 2008''

Revision as of 12:36, 27 July 2008

Image:2yqh.png

Template:STRUCTURE 2yqh

Crystal structure of uridine-diphospho-N-acetylglucosamine pyrophosphorylase from Candida albicans, in the substrate-binding form

Template:ABSTRACT PUBMED 17392279

About this Structure

2YQH is a Single protein structure of sequence from Candida albicans. Full crystallographic information is available from OCA.

Reference

Crystal structure of uridine-diphospho-N-acetylglucosamine pyrophosphorylase from Candida albicans and catalytic reaction mechanism., Maruyama D, Nishitani Y, Nonaka T, Kita A, Fukami TA, Mio T, Yamada-Okabe H, Yamada-Okabe T, Miki K, J Biol Chem. 2007 Jun 8;282(23):17221-30. Epub 2007 Mar 28. PMID:17392279

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