2oz4

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{{STRUCTURE_2oz4| PDB=2oz4 | SCENE= }}
{{STRUCTURE_2oz4| PDB=2oz4 | SCENE= }}
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'''Structural Plasticity in IgSF Domain 4 of ICAM-1 Mediates Cell Surface Dimerization'''
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===Structural Plasticity in IgSF Domain 4 of ICAM-1 Mediates Cell Surface Dimerization===
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==Overview==
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The Ig superfamily (IgSF) intercellular adhesion molecule-1 (ICAM-1) equilibrates between monomeric and dimeric forms on the cell surface, and dimerization enhances cell adhesion. A crystal structure of ICAM-1 IgSF domains (D) 3-5 revealed a unique dimerization interface in which D4s of two protomers fuse through edge beta-strands to form a single super beta-sandwich domain. Here, we describe a crystal structure at 2.7-A resolution of monomeric ICAM-1 D3-D5, stabilized by the monomer-specific Fab CA7. CA7 binds to D5 in a region that is buried in the dimeric interface and is distal from the dimerization site in D4. In monomeric ICAM-1 D3-D5, a 16-residue loop in D4 that is disordered in the dimeric structure could clearly be traced as a BC loop, a short C strand, and a CE meander with a cis-Pro followed by a solvent-exposed, flexible four-residue region. Deletions of 6 or 10 residues showed that the C-strand is essential for monomer stability, whereas a distinct six-residue deletion showed little contribution of the CE meander. Mutation of two inward-pointing Leu residues in edge beta-strand E to Lys increased monomer stability, confirming the hypothesis that inward-pointing charged side chains on edge beta-strands are an important design feature to prevent beta-supersheet formation. Overall, the studies reveal that monomer-dimer transition is associated with a surprisingly large, physiologically relevant, IgSF domain rearrangement.
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==Disease==
==Disease==
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[[Category: Igsf domain]]
[[Category: Igsf domain]]
[[Category: Structural plasticity]]
[[Category: Structural plasticity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:36:24 2008''

Revision as of 12:36, 27 July 2008

Image:2oz4.png

Template:STRUCTURE 2oz4

Contents

Structural Plasticity in IgSF Domain 4 of ICAM-1 Mediates Cell Surface Dimerization

Template:ABSTRACT PUBMED 17881562

Disease

Known disease associated with this structure: Malaria, cerebral, susceptibility to OMIM:[147840]

About this Structure

2OZ4 is a Single protein structure of sequence from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural plasticity in Ig superfamily domain 4 of ICAM-1 mediates cell surface dimerization., Chen X, Kim TD, Carman CV, Mi LZ, Song G, Springer TA, Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15358-63. Epub 2007 Sep 19. PMID:17881562

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