1xcg

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{{STRUCTURE_1xcg| PDB=1xcg | SCENE= }}
{{STRUCTURE_1xcg| PDB=1xcg | SCENE= }}
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'''Crystal Structure of Human RhoA in complex with DH/PH fragment of PDZRHOGEF'''
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===Crystal Structure of Human RhoA in complex with DH/PH fragment of PDZRHOGEF===
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==Overview==
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Calcium sensitization in smooth muscle is mediated by the RhoA GTPase, activated by hitherto unspecified nucleotide exchange factors (GEFs) acting downstream of Galphaq/Galpha(12/13) trimeric G proteins. Here, we show that at least one potential GEF, the PDZRhoGEF, is present in smooth muscle, and its isolated DH/PH fragment induces calcium sensitization in the absence of agonist-mediated signaling. In vitro, the fragment shows high selectivity for the RhoA GTPase. Full-length fragment is required for the nucleotide exchange, as the isolated DH domain enhances it only marginally. We crystallized the DH/PH fragment of PDZRhoGEF in complex with nonprenylated human RhoA and determined the structure at 2.5 A resolution. The refined molecular model reveals that the mutual disposition of the DH and PH domains is significantly different from other previously described complexes involving DH/PH tandems, and that the PH domain interacts with RhoA in a unique mode. The DH domain makes several specific interactions with RhoA residues not conserved among other Rho family members, suggesting the molecular basis for the observed specificity.
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{{ABSTRACT_PUBMED_15530360}}
==About this Structure==
==About this Structure==
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[[Category: Regulation of rhoa gtpase]]
[[Category: Regulation of rhoa gtpase]]
[[Category: X-ray crystallography]]
[[Category: X-ray crystallography]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:39:14 2008''

Revision as of 12:39, 27 July 2008

Image:1xcg.png

Template:STRUCTURE 1xcg

Crystal Structure of Human RhoA in complex with DH/PH fragment of PDZRHOGEF

Template:ABSTRACT PUBMED 15530360

About this Structure

1XCG is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The crystal structure of RhoA in complex with the DH/PH fragment of PDZRhoGEF, an activator of the Ca(2+) sensitization pathway in smooth muscle., Derewenda U, Oleksy A, Stevenson AS, Korczynska J, Dauter Z, Somlyo AP, Otlewski J, Somlyo AV, Derewenda ZS, Structure. 2004 Nov;12(11):1955-65. PMID:15530360

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