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1z2j

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[[Image:1z2j.gif|left|200px]]
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{{STRUCTURE_1z2j| PDB=1z2j | SCENE= }}
{{STRUCTURE_1z2j| PDB=1z2j | SCENE= }}
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'''Solution structure of the HIV-1 frameshift inducing element'''
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===Solution structure of the HIV-1 frameshift inducing element===
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==Overview==
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Expression of the HIV reverse transcriptase and other essential viral enzymes requires a -1 translational frameshift. The frameshift event is induced by two highly conserved RNA elements within the HIV-1 mRNA: a UUUUUUA heptamer known as the slippery sequence, and a downstream RNA structure. Here, we report structural and thermodynamic evidence that the HIV-1 frameshift site RNA forms a stem-loop and lower helix separated by a three-purine bulge. We have determined the structure of the 45 nucleotide frameshift site RNA using multidimensional heteronuclear nuclear magnetic resonance (NMR) methods. The upper helix is highly thermostable (T(m)&gt;90 degrees C), forming 11 Watson-Crick base-pairs capped by a stable ACAA tetraloop. The eight base-pair lower helix was found to be only moderately stable (T(m)=47 degrees C). A three-purine bulge separates the highly stable upper helix from the lower helix. Base stacking in the bulge forms a wedge, introducing a 60 degrees bend between the helices. Interestingly, this bend is similar to those seen in a number of frameshift inducing pseudoknots for which structures have been solved. The lower helix must denature to allow the ribosome access to the slippery site, but likely functions as a positioning element that enhances frameshift efficiency.
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(as it appears on PubMed at http://www.pubmed.gov), where 15927637 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15927637}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z2J OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z2J OCA].
==Reference==
==Reference==
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[[Category: Stem-loop]]
[[Category: Stem-loop]]
[[Category: Tetraloop]]
[[Category: Tetraloop]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:06:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:44:00 2008''

Revision as of 12:44, 27 July 2008

Image:1z2j.png

Template:STRUCTURE 1z2j

Solution structure of the HIV-1 frameshift inducing element

Template:ABSTRACT PUBMED 15927637

About this Structure

Full experimental information is available from OCA.

Reference

Solution structure and thermodynamic investigation of the HIV-1 frameshift inducing element., Staple DW, Butcher SE, J Mol Biol. 2005 Jun 24;349(5):1011-23. Epub 2005 Apr 1. PMID:15927637

Page seeded by OCA on Sun Jul 27 15:44:00 2008

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