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| | {{STRUCTURE_1z2j| PDB=1z2j | SCENE= }} | | {{STRUCTURE_1z2j| PDB=1z2j | SCENE= }} |
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| - | '''Solution structure of the HIV-1 frameshift inducing element'''
| + | ===Solution structure of the HIV-1 frameshift inducing element=== |
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| - | ==Overview==
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| - | Expression of the HIV reverse transcriptase and other essential viral enzymes requires a -1 translational frameshift. The frameshift event is induced by two highly conserved RNA elements within the HIV-1 mRNA: a UUUUUUA heptamer known as the slippery sequence, and a downstream RNA structure. Here, we report structural and thermodynamic evidence that the HIV-1 frameshift site RNA forms a stem-loop and lower helix separated by a three-purine bulge. We have determined the structure of the 45 nucleotide frameshift site RNA using multidimensional heteronuclear nuclear magnetic resonance (NMR) methods. The upper helix is highly thermostable (T(m)>90 degrees C), forming 11 Watson-Crick base-pairs capped by a stable ACAA tetraloop. The eight base-pair lower helix was found to be only moderately stable (T(m)=47 degrees C). A three-purine bulge separates the highly stable upper helix from the lower helix. Base stacking in the bulge forms a wedge, introducing a 60 degrees bend between the helices. Interestingly, this bend is similar to those seen in a number of frameshift inducing pseudoknots for which structures have been solved. The lower helix must denature to allow the ribosome access to the slippery site, but likely functions as a positioning element that enhances frameshift efficiency.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_15927637}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15927637 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15927637}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z2J OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z2J OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Stem-loop]] | | [[Category: Stem-loop]] |
| | [[Category: Tetraloop]] | | [[Category: Tetraloop]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:06:32 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:44:00 2008'' |
Revision as of 12:44, 27 July 2008
Template:STRUCTURE 1z2j
Solution structure of the HIV-1 frameshift inducing element
Template:ABSTRACT PUBMED 15927637
About this Structure
Full experimental information is available from OCA.
Reference
Solution structure and thermodynamic investigation of the HIV-1 frameshift inducing element., Staple DW, Butcher SE, J Mol Biol. 2005 Jun 24;349(5):1011-23. Epub 2005 Apr 1. PMID:15927637
Page seeded by OCA on Sun Jul 27 15:44:00 2008
