1omo

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[[Image:1omo.gif|left|200px]]
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{{STRUCTURE_1omo| PDB=1omo | SCENE= }}
{{STRUCTURE_1omo| PDB=1omo | SCENE= }}
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'''alanine dehydrogenase dimer w/bound NAD (archaeal)'''
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===alanine dehydrogenase dimer w/bound NAD (archaeal)===
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==Overview==
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The hyperthermophilic archaeon Archaeoglobus fulgidus contains an L-Ala dehydrogenase (AlaDH, EC 1.4.1.1) that is not homologous to known bacterial dehydrogenases and appears to represent a previously unrecognized archaeal group of NAD-dependent dehydrogenases. The gene (Genbank; TIGR AF1665) was annotated initially as an ornithine cyclodeaminase (OCD) on the basis of strong homology with the mu crystallin/OCD protein family. We report the structure of the NAD-bound AF1665 AlaDH (AF-AlaDH) at 2.3 A in a C2 crystal form with the 70 kDa dimer in the asymmetric unit, as the first structural representative of this family. Consistent with its lack of homology to bacterial AlaDH proteins, which are mostly hexameric, the archaeal dimer has a novel structure. Although both types of AlaDH enzyme include a Rossmann-type NAD-binding domain, the arrangement of strands in the C-terminal half of this domain is novel, and the other (catalytic) domain in the archaeal protein has a new fold. The active site presents a cluster of conserved Arg and Lys side-chains over the pro-R face of the cofactor. In addition, the best ordered of the 338 water molecules in the structure is positioned well for mechanistic interaction. The overall structure and active site are compared with other dehydrogenases, including the AlaDH from Phormidium lapideum. Implications for the catalytic mechanism and for the structures of homologs are considered. The archaeal AlaDH represents an ancient and previously undescribed subclass of Rossmann-fold proteins that includes bacterial ornithine and lysine cyclodeaminases, marsupial lens proteins and, in man, a thyroid hormone-binding protein that exhibits 30% sequence identity with AF1665.
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(as it appears on PubMed at http://www.pubmed.gov), where 15313611 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15313611}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structure of alanine dehydrogenase from Archaeoglobus: active site analysis and relation to bacterial cyclodeaminases and mammalian mu crystallin., Gallagher DT, Monbouquette HG, Schroder I, Robinson H, Holden MJ, Smith NN, J Mol Biol. 2004 Sep 3;342(1):119-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15313611 15313611]
Structure of alanine dehydrogenase from Archaeoglobus: active site analysis and relation to bacterial cyclodeaminases and mammalian mu crystallin., Gallagher DT, Monbouquette HG, Schroder I, Robinson H, Holden MJ, Smith NN, J Mol Biol. 2004 Sep 3;342(1):119-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15313611 15313611]
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Crystallization and phasing of alanine dehydrogenase from Archaeoglobus fulgidus., Smith N, Mayhew M, Robinson H, Heroux A, Charlton D, Holden MJ, Gallagher DT, Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2328-31. Epub 2003, Nov 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14646110 14646110]
[[Category: Alanine dehydrogenase]]
[[Category: Alanine dehydrogenase]]
[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
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[[Category: Rossmann-fold nad domain]]
[[Category: Rossmann-fold nad domain]]
[[Category: Two-domain]]
[[Category: Two-domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:02:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:52:12 2008''

Revision as of 12:52, 27 July 2008

Image:1omo.png

Template:STRUCTURE 1omo

alanine dehydrogenase dimer w/bound NAD (archaeal)

Template:ABSTRACT PUBMED 15313611

About this Structure

1OMO is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.

Reference

Structure of alanine dehydrogenase from Archaeoglobus: active site analysis and relation to bacterial cyclodeaminases and mammalian mu crystallin., Gallagher DT, Monbouquette HG, Schroder I, Robinson H, Holden MJ, Smith NN, J Mol Biol. 2004 Sep 3;342(1):119-30. PMID:15313611

Crystallization and phasing of alanine dehydrogenase from Archaeoglobus fulgidus., Smith N, Mayhew M, Robinson H, Heroux A, Charlton D, Holden MJ, Gallagher DT, Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2328-31. Epub 2003, Nov 27. PMID:14646110

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