1z1d

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[[Image:1z1d.gif|left|200px]]
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{{Seed}}
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[[Image:1z1d.png|left|200px]]
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{{STRUCTURE_1z1d| PDB=1z1d | SCENE= }}
{{STRUCTURE_1z1d| PDB=1z1d | SCENE= }}
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'''Structural Model for the interaction between RPA32 C-terminal domain and SV40 T antigen origin binding domain.'''
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===Structural Model for the interaction between RPA32 C-terminal domain and SV40 T antigen origin binding domain.===
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==Overview==
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Simian virus 40 (SV40) provides a model system for the study of eukaryotic DNA replication, in which the viral protein, large T antigen (Tag), marshals human proteins to replicate the viral minichromosome. SV40 replication requires interaction of Tag with the host single-stranded DNA-binding protein, replication protein A (hRPA). The C-terminal domain of the hRPA32 subunit (RPA32C) facilitates initiation of replication, but whether it interacts with Tag is not known. Affinity chromatography and NMR revealed physical interaction between hRPA32C and the Tag origin DNA-binding domain, and a structural model of the complex was determined. Point mutations were then designed to reverse charges in the binding sites, resulting in substantially reduced binding affinity. Corresponding mutations introduced into intact hRPA impaired initiation of replication and primosome activity, implying that this interaction has a critical role in assembly and progression of the SV40 replisome.
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The line below this paragraph, {{ABSTRACT_PUBMED_15793585}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15793585 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15793585}}
==About this Structure==
==About this Structure==
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1Z1D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1D OCA].
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1Z1D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1D OCA].
==Reference==
==Reference==
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[[Category: Protein-protein complex]]
[[Category: Protein-protein complex]]
[[Category: Winged helix-turn-helix motif]]
[[Category: Winged helix-turn-helix motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:04:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:09:48 2008''

Revision as of 13:09, 27 July 2008

Image:1z1d.png

Template:STRUCTURE 1z1d

Structural Model for the interaction between RPA32 C-terminal domain and SV40 T antigen origin binding domain.

Template:ABSTRACT PUBMED 15793585

About this Structure

1Z1D is a Protein complex structure of sequences from Homo sapiens and Simian virus 40. Full experimental information is available from OCA.

Reference

Insights into hRPA32 C-terminal domain--mediated assembly of the simian virus 40 replisome., Arunkumar AI, Klimovich V, Jiang X, Ott RD, Mizoue L, Fanning E, Chazin WJ, Nat Struct Mol Biol. 2005 Apr;12(4):332-9. Epub 2005 Mar 27. PMID:15793585

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