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1rjv

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[[Image:1rjv.gif|left|200px]]
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{{STRUCTURE_1rjv| PDB=1rjv | SCENE= }}
{{STRUCTURE_1rjv| PDB=1rjv | SCENE= }}
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'''Solution Structure of Human alpha-Parvalbumin refined with a paramagnetism-based strategy'''
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===Solution Structure of Human alpha-Parvalbumin refined with a paramagnetism-based strategy===
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==Overview==
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In the frame of a research aimed at the detailed structural characterization of human calcium-binding proteins of the EF-hand family, the solution structure of human alpha-parvalbumin has been solved by NMR and refined with the help of substitution of the Ca(2+) ion in the EF site with the paramagnetic Dy(3+) ion. A simple (1)H-(15)N HSQC spectrum allowed the NH assignments based on the properties of Dy(3+). This allowed us to exploit pseudocontact shifts and residual dipolar couplings for solution structure refinement. The backbone and heavy atom RMSD are 0.55 +/- 0.08 and 1.02 +/- 0.08 A, respectively, and decrease to 0.39 +/- 0.05 and 0.90 +/- 0.06 A upon refinement with paramagnetism-based restraints. The RMSD for the metal itself in the EF site in the refined structure is 0.26 +/- 0.12 A. Backbone NH R(1), R(2), and NOE measured at two temperatures show the protein to be relatively rigid. The NH orientations are well determined by the paramagnetism-based restraints. This allows us to detect small but significant local structural differences with the orthologue protein from rat, whose X-ray structure is available at 2.0 A resolution. All differences are related to local changes in the amino acidic composition.
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(as it appears on PubMed at http://www.pubmed.gov), where 15122922 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15122922}}
==About this Structure==
==About this Structure==
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1RJV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJV OCA].
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1RJV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJV OCA].
==Reference==
==Reference==
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[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Structural proteomics in europe]]
[[Category: Structural proteomics in europe]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 13 08:10:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:25:13 2008''

Revision as of 13:25, 27 July 2008

Image:1rjv.png

Template:STRUCTURE 1rjv

Solution Structure of Human alpha-Parvalbumin refined with a paramagnetism-based strategy

Template:ABSTRACT PUBMED 15122922

About this Structure

1RJV is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Paramagnetism-based refinement strategy for the solution structure of human alpha-parvalbumin., Baig I, Bertini I, Del Bianco C, Gupta YK, Lee YM, Luchinat C, Quattrone A, Biochemistry. 2004 May 11;43(18):5562-73. PMID:15122922

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