1kcf
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1kcf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kcf, resolution 2.30Å" /> '''Crystal Structure of...)
Next diff →
Revision as of 16:56, 20 November 2007
|
Crystal Structure of the Yeast Mitochondrial Holliday Junction Resolvase, Ydc2
Overview
Resolution of Holliday junctions into separate DNA duplexes requires, enzymatic cleavage of an equivalent strand from each contributing duplex, at or close to the point of strand exchange. Diverse Holliday, junction-resolving enzymes have been identified in bacteria, bacteriophages, archaea and pox viruses, but the only eukaryotic examples, identified so far are those from fungal mitochondria. We have now, determined the crystal structure of Ydc2 (also known as SpCce1), a, Holliday junction resolvase from the fission yeast Schizosaccharomyces, pombe that is involved in the maintenance of mitochondrial DNA. This first, structure of a eukaryotic Holliday junction resolvase confirms a distant, evolutionary relationship to the bacterial RuvC family, but reveals, structural features which are unique to the eukaryotic enzymes. Detailed, analysis of the dimeric structure suggests mechanisms for junction, isomerization and communication between the two active sites, and together, with site-directed mutagenesis identifies residues involved in catalysis.
About this Structure
1KCF is a Single protein structure of sequence from Schizosaccharomyces pombe with SO4 as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the fission yeast mitochondrial Holliday junction resolvase Ydc2., Ceschini S, Keeley A, McAlister MS, Oram M, Phelan J, Pearl LH, Tsaneva IR, Barrett TE, EMBO J. 2001 Dec 3;20(23):6601-11. PMID:11726496
Page seeded by OCA on Tue Nov 20 19:03:34 2007
