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2v8i

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[[Image:2v8i.jpg|left|200px]]
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{{Seed}}
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[[Image:2v8i.png|left|200px]]
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{{STRUCTURE_2v8i| PDB=2v8i | SCENE= }}
{{STRUCTURE_2v8i| PDB=2v8i | SCENE= }}
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'''STRUCTURE OF A FAMILY 2 PECTATE LYASE IN A NATIVE FORM'''
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===STRUCTURE OF A FAMILY 2 PECTATE LYASE IN A NATIVE FORM===
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==Overview==
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The family 2 pectate lyase from Yersinia enterocolitica (YePL2A), solved to 1.5A, reveals it to be the first prokaryotic protein reported to display the rare (alpha/alpha)(7) barrel fold. In addition to its apo form, we have also determined the structure of a metal-bound form of YePL2A (to 2.0A) and a trigalacturonic acid-bound substrate complex (to 2.1A) Although its fold is rare, the catalytic center of YePL2A can be superimposed with structurally unrelated families, underlining the conserved catalytic amino acid architecture of the beta-elimination mechanism. In addition to its overall structure, YePL2A also has two other unique features: 1) it utilizes a metal atom other than calcium for catalysis, and 2) its Bronstead base is in an alternate conformation and directly interacts with the uronate group of the substrate.
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The line below this paragraph, {{ABSTRACT_PUBMED_17881361}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17881361 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17881361}}
==About this Structure==
==About this Structure==
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[[Category: Pectin degradation]]
[[Category: Pectin degradation]]
[[Category: Periplasm]]
[[Category: Periplasm]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 18:22:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:29:24 2008''

Revision as of 13:29, 27 July 2008

Image:2v8i.png

Template:STRUCTURE 2v8i

STRUCTURE OF A FAMILY 2 PECTATE LYASE IN A NATIVE FORM

Template:ABSTRACT PUBMED 17881361

About this Structure

2V8I is a Single protein structure of sequence from Yersinia enterocolitica. Full crystallographic information is available from OCA.

Reference

A family 2 pectate lyase displays a rare fold and transition metal-assisted beta-elimination., Abbott DW, Boraston AB, J Biol Chem. 2007 Nov 30;282(48):35328-36. Epub 2007 Sep 19. PMID:17881361

Page seeded by OCA on Sun Jul 27 16:29:24 2008

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