1qkz

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[[Image:1qkz.gif|left|200px]]
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{{Seed}}
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[[Image:1qkz.png|left|200px]]
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{{STRUCTURE_1qkz| PDB=1qkz | SCENE= }}
{{STRUCTURE_1qkz| PDB=1qkz | SCENE= }}
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'''FAB FRAGMENT (MN14C11.6) IN COMPLEX WITH A PEPTIDE ANTIGEN DERIVED FROM NEISSERIA MENINGITIDIS P1.7 SEROSUBTYPE ANTIGEN AND DOMAIN II FROM STREPTOCOCCAL PROTEIN G'''
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===FAB FRAGMENT (MN14C11.6) IN COMPLEX WITH A PEPTIDE ANTIGEN DERIVED FROM NEISSERIA MENINGITIDIS P1.7 SEROSUBTYPE ANTIGEN AND DOMAIN II FROM STREPTOCOCCAL PROTEIN G===
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==Overview==
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Many pathogens present highly variable surface proteins to their host as a means of evading immune responses. The structure of a peptide antigen corresponding to the subtype P1.7 variant of the porin PorA from the human pathogen Neisseria meningitidis was determined by solution of the X-ray crystal structure of the ternary complex of the peptide (ANGGASGQVK) in complex with a Fab fragment and a domain from streptococcal protein G to 1.95 A resolution. The peptide adopted a beta-hairpin structure with a type I beta-turn between residues Gly4P and Gly7P, the conformation of the peptide being further stabilised by a pair of hydrogen bonds from the side-chain of Asn2P to main-chain atoms in Val9P. The antigen binding site within the Fab formed a distinct crevice lined by a high proportion of apolar amino acids. Recognition was supplemented by hydrogen bonds from heavy chain residues Thr50H, Asp95H, Leu97H and Tyr100H to main-chain and side-chain atoms in the peptide. Complementarity-determining region (CDR) 3 of the heavy chain was responsible for approximately 50 % of the buried surface area formed by peptide-Fab binding, with the remainder made up from CDRs 1 and 3 of the light chain and CDRs 1 and 2 of the heavy chain. Knowledge of the structures of variable surface antigens such as PorA is an essential prerequisite to a molecular understanding of antigenic variation and its implications for vaccine design.
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(as it appears on PubMed at http://www.pubmed.gov), where 10512717 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10512717}}
==About this Structure==
==About this Structure==
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[[Category: Pora]]
[[Category: Pora]]
[[Category: Porin]]
[[Category: Porin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:24:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:35:25 2008''

Revision as of 13:35, 27 July 2008

Image:1qkz.png

Template:STRUCTURE 1qkz

FAB FRAGMENT (MN14C11.6) IN COMPLEX WITH A PEPTIDE ANTIGEN DERIVED FROM NEISSERIA MENINGITIDIS P1.7 SEROSUBTYPE ANTIGEN AND DOMAIN II FROM STREPTOCOCCAL PROTEIN G

Template:ABSTRACT PUBMED 10512717

About this Structure

1QKZ is a Protein complex structure of sequences from Mus musculus, Neisseria meningitidis and Streptococcus. Full crystallographic information is available from OCA.

Reference

Crystal structure of an Fab fragment in complex with a meningococcal serosubtype antigen and a protein G domain., Derrick JP, Maiden MC, Feavers IM, J Mol Biol. 1999 Oct 15;293(1):81-91. PMID:10512717

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