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1rbb

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[[Image:1rbb.gif|left|200px]]
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{{STRUCTURE_1rbb| PDB=1rbb | SCENE= }}
{{STRUCTURE_1rbb| PDB=1rbb | SCENE= }}
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'''THE CRYSTAL STRUCTURE OF RIBONUCLEASE B AT 2.5-ANGSTROMS RESOLUTION'''
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===THE CRYSTAL STRUCTURE OF RIBONUCLEASE B AT 2.5-ANGSTROMS RESOLUTION===
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==Overview==
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The glycosylated form of bovine pancreatic ribonuclease, RNase B, was crystallized from polyethylene glycol 4000 at low ionic strength in space group C2 with unit cell dimensions of a = 101.81 A, b = 33.36 A, c = 73.60 A, and beta = 90.4 degrees. The crystals, which contained two independent molecules of RNase B as the asymmetric unit, were solved by a combination of multiple isomorphous replacement and molecular replacement approaches. The structures of the two molecules were refined to 2.5-A resolution and a conventional R factor of 0.22 using a constrained-restrained least squares procedure (CORELS). Complexes were also investigated of RNase B plus ruthenium pentaamine and between RNase B and a substrate analogue iodouridine. The polypeptide backbones of the two molecules of RNase B in the asymmetric unit were found to be statistically identical and their differences from RNase A to be statistically insignificant. The carbohydrate chains of both molecules extended into solvent cavities in the crystal lattice and appear to be disordered for the most part. The oligosaccharides appear to exert no influence on the structure of the protein. Iodouridine was observed to bind identically in the pyrimidine site of both RNase B molecules and in a way apparently the same as that previously observed for RNase A. Ruthenium pentaamine bound at histidine 105 of both RNase B molecules in the asymmetric unit, but at a number of secondary sites as well. An array of bound ions was observed by Fo-Fc difference Fourier syntheses. These ions were proximal to lysine and arginine residues at the surface of the proteins while a pair of strong ion binding sites were seen to fall exactly in the active site clefts of both RNase B molecules in the asymmetric unit.
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The line below this paragraph, {{ABSTRACT_PUBMED_3680242}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 3680242 is the PubMed ID number.
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{{ABSTRACT_PUBMED_3680242}}
==About this Structure==
==About this Structure==
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[[Category: McPherson, A.]]
[[Category: McPherson, A.]]
[[Category: Williams, R L.]]
[[Category: Williams, R L.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:18:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:37:34 2008''

Revision as of 13:37, 27 July 2008

Image:1rbb.png

Template:STRUCTURE 1rbb

THE CRYSTAL STRUCTURE OF RIBONUCLEASE B AT 2.5-ANGSTROMS RESOLUTION

Template:ABSTRACT PUBMED 3680242

About this Structure

1RBB is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

The crystal structure of ribonuclease B at 2.5-A resolution., Williams RL, Greene SM, McPherson A, J Biol Chem. 1987 Nov 25;262(33):16020-31. PMID:3680242

Page seeded by OCA on Sun Jul 27 16:37:34 2008

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