This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1ukp

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1ukp.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1ukp.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1ukp| PDB=1ukp | SCENE= }}
{{STRUCTURE_1ukp| PDB=1ukp | SCENE= }}
-
'''Crystal structure of soybean beta-amylase mutant substituted at surface region'''
+
===Crystal structure of soybean beta-amylase mutant substituted at surface region===
-
==Overview==
+
<!--
-
In spite of the high similarity of amino acid sequence and three-dimensional structure between soybean beta-amylase (SBA) and sweet potato beta-amylase (SPB), their quaternary structure is quite different, being a monomer in SBA and a tetramer in SPB. Because most of the differences in amino acid sequences are found in the surface region, we tested the tetramerization of SBA by examining mutations of residues located at the surface. We designed the SBA tetramer using the SPB tetramer structure as a model and calculating the change of accessible surface area (DeltaASA) for each residue in order to select sites for the mutation. Two different mutant genes encoding SB3 (D374Y/L481R/P487D) and SB4 (K462S added to SB3), were constructed for expression in Escherichia coli and the recombinant proteins were purified. They existed as a monomer in solution, but gave completely different crystals from the native SBA. The asymmetric unit of the mutants contains four molecules, while that of native SBA contains one. The interactions of the created interfaces revealed that there were more intermolecular interactions in the SB3 than in the SB4 tetramer. The substituted charged residues on the surface are involved in interactions with adjacent molecules in a different way, forming a new crystal packing pattern.
+
The line below this paragraph, {{ABSTRACT_PUBMED_14631070}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 14631070 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_14631070}}
==About this Structure==
==About this Structure==
Line 27: Line 31:
[[Category: Mikami, B.]]
[[Category: Mikami, B.]]
[[Category: Utsumi, S.]]
[[Category: Utsumi, S.]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:21:45 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:39:34 2008''

Revision as of 13:39, 27 July 2008

Image:1ukp.png

Template:STRUCTURE 1ukp

Crystal structure of soybean beta-amylase mutant substituted at surface region

Template:ABSTRACT PUBMED 14631070

About this Structure

1UKP is a Single protein structure of sequence from Glycine max. Full crystallographic information is available from OCA.

Reference

Change in the crystal packing of soybean beta-amylase mutants substituted at a few surface amino acid residues., Kang YN, Adachi M, Mikami B, Utsumi S, Protein Eng. 2003 Nov;16(11):809-17. PMID:14631070

Page seeded by OCA on Sun Jul 27 16:39:34 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ukp"
Personal tools