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| - | [[Image:1o91.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1o91| PDB=1o91 | SCENE= }} | | {{STRUCTURE_1o91| PDB=1o91 | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF A COLLAGEN VIII NC1 DOMAIN TRIMER'''
| + | ===CRYSTAL STRUCTURE OF A COLLAGEN VIII NC1 DOMAIN TRIMER=== |
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| - | ==Overview==
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| - | Collagen VIII is a major component of Descemet's membrane and is also found in vascular subendothelial matrices. The C-terminal non-collagenous domain (NC1) domain of collagen VIII, which is a member of the C1q-like protein family, forms a stable trimer and is thought to direct the assembly of the collagen triple helix, as well as polygonal supramolecular structures. We have solved the crystal structure of the mouse alpha1(VIII)(3) NC1 domain trimer at 1.9 A resolution. Each subunit of the intimate NC1 trimer consists of a ten-stranded beta-sandwich. The surface of the collagen VIII NC1 trimer presents three strips of partially exposed aromatic residues shown to interact with the non-ionic detergent CHAPS, which are likely to be involved in supramolecular assemblies. Equivalent strips exist in the NC1 domain of the closely related collagen X, suggesting a conserved assembly mechanism. Surprisingly, the collagen VIII NC1 trimer lacks the buried calcium cluster of the collagen X NC1 trimer. The mouse alpha1(VIII) and alpha2(VIII) NC1 domains are 71.5% identical in sequence, with the differences being concentrated on the NC1 trimer surface. A few non-conservative substitutions map to the subunit interfaces near the surface, but it is not obvious from the structure to what extent they determine the preferred assembly of collagen VIII alpha1 and alpha2 chains into homotrimers.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_12782141}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 12782141 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_12782141}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Collagen]] | | [[Category: Collagen]] |
| | [[Category: Extracellular matrix]] | | [[Category: Extracellular matrix]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:32:27 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:47:24 2008'' |
Revision as of 13:47, 27 July 2008
Template:STRUCTURE 1o91
CRYSTAL STRUCTURE OF A COLLAGEN VIII NC1 DOMAIN TRIMER
Template:ABSTRACT PUBMED 12782141
About this Structure
1O91 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the collagen alpha1(VIII) NC1 trimer., Kvansakul M, Bogin O, Hohenester E, Yayon A, Matrix Biol. 2003 Apr;22(2):145-52. PMID:12782141
Page seeded by OCA on Sun Jul 27 16:47:24 2008
