2p89

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{{STRUCTURE_2p89| PDB=2p89 | SCENE= }}
{{STRUCTURE_2p89| PDB=2p89 | SCENE= }}
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'''Solution structure of the 3' pseudouridyation pocket of U65 snoRNA with bound substrate'''
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===Solution structure of the 3' pseudouridyation pocket of U65 snoRNA with bound substrate===
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==Overview==
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During the biogenesis of eukaryotic ribosomal RNA (rRNA) and spliceosomal small nuclear RNA (snRNA), uridines at specific sites are converted to pseudouridines by H/ACA ribonucleoprotein particles (RNPs). Each H/ACA RNP contains a substrate-specific H/ACA RNA and four common proteins, the pseudouridine synthase Cbf5, Nop10, Gar1, and Nhp2. The H/ACA RNA contains at least one pseudouridylation (psi) pocket, which is complementary to the sequences flanking the target uridine. In this article, we show structural evidence that the psi pocket can form the predicted base pairs with substrate RNA in the absence of protein components. We report the solution structure of the complex between an RNA hairpin derived from the 3' psi pocket of human U65 H/ACA small nucleolar RNA (snoRNA) and the substrate rRNA. The snoRNA-rRNA substrate complex has a unique structure with two offset parallel pairs of stacked helices and two unusual intermolecular three-way junctions, which together organize the substrate for docking into the active site of Cbf5. The substrate RNA interacts on one face of the snoRNA in the complex, forming a structure that easily could be accommodated in the H/ACA RNP, and explains how successive substrate RNAs could be loaded onto and unloaded from the H/ACA RNA in the RNP.
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(as it appears on PubMed at http://www.pubmed.gov), where 17412831 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17412831}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P89 OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P89 OCA].
==Reference==
==Reference==
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[[Category: Rna-rna complex]]
[[Category: Rna-rna complex]]
[[Category: Three-way junction]]
[[Category: Three-way junction]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:35:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:49:25 2008''

Revision as of 13:49, 27 July 2008

Image:2p89.png

Template:STRUCTURE 2p89

Solution structure of the 3' pseudouridyation pocket of U65 snoRNA with bound substrate

Template:ABSTRACT PUBMED 17412831

About this Structure

Full experimental information is available from OCA.

Reference

H/ACA small nucleolar RNA pseudouridylation pockets bind substrate RNA to form three-way junctions that position the target U for modification., Wu H, Feigon J, Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6655-60. Epub 2007 Apr 5. PMID:17412831

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