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1nly

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{{STRUCTURE_1nly| PDB=1nly | SCENE= }}
{{STRUCTURE_1nly| PDB=1nly | SCENE= }}
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'''Crystal structure of the traffic ATPase of the Helicobacter pylori type IV secretion system in complex with ATPgammaS'''
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===Crystal structure of the traffic ATPase of the Helicobacter pylori type IV secretion system in complex with ATPgammaS===
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==Overview==
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The coupling of ATP binding/hydrolysis to macromolecular secretion systems is crucial to the pathogenicity of Gram-negative bacteria. We reported previously the structure of the ADP-bound form of the hexameric traffic VirB11 ATPase of the Helicobacter pylori type IV secretion system (named HP0525), and proposed that it functions as a gating molecule at the inner membrane, cycling through closed and open forms regulated by ATP binding/hydrolysis. Here, we combine crystal structures with analytical ultracentrifugation experiments to show that VirB11 ATPases indeed function as dynamic hexameric assemblies. In the absence of nucleotide, the N-terminal domains exhibit a collection of rigid-body conformations. Nucleotide binding 'locks' the hexamer into a symmetric and compact structure. We propose that VirB11s use the mechanical leverage generated by such nucleotide-dependent conformational changes to facilitate the export of substrates or the assembly of the type IV secretion apparatus. Biochemical characterization of mutant forms of HP0525 coupled with electron microscopy and in vivo assays support such hypothesis, and establish the relevance of VirB11s ATPases as drug targets against pathogenic bacteria.
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{{ABSTRACT_PUBMED_12727865}}
==About this Structure==
==About this Structure==
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[[Category: Hp0525]]
[[Category: Hp0525]]
[[Category: Virb11 atpase]]
[[Category: Virb11 atpase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:41:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:51:57 2008''

Revision as of 13:52, 27 July 2008

Image:1nly.png

Template:STRUCTURE 1nly

Crystal structure of the traffic ATPase of the Helicobacter pylori type IV secretion system in complex with ATPgammaS

Template:ABSTRACT PUBMED 12727865

About this Structure

1NLY is a Single protein structure of sequence from Helicobacter pylori 26695. Full crystallographic information is available from OCA.

Reference

VirB11 ATPases are dynamic hexameric assemblies: new insights into bacterial type IV secretion., Savvides SN, Yeo HJ, Beck MR, Blaesing F, Lurz R, Lanka E, Buhrdorf R, Fischer W, Haas R, Waksman G, EMBO J. 2003 May 1;22(9):1969-80. PMID:12727865

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