1kf8

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Revision as of 17:02, 20 November 2007

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spinqualitylabelsall models 1kf8, resolution 1.15Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Atomic resolution structure of RNase A at pH 8.8

Overview

The diffraction pattern of protein crystals extending to atomic resolution, guarantees a very accurate picture of the molecular structure and enables, the study of subtle phenomena related to protein functionality. Six, structures of bovine pancreatic ribonuclease at the pH* values 5.2, 5.9, 6.3, 7.1, 8.0 and 8.8 and at resolution limits in the range 1.05-1.15A, have been refined. An overall description of the six structures and, several aspects, mainly regarding pH-triggered conformational changes, are, described here. Since subtle variations were expected, a thorough, validation assessment of the six refined models was first carried out., Some stereochemical parameters, such as the N[bond]C(alpha)[bond]C angle, and the pyramidalization at the carbonyl C atoms, indicate that the, standard target values and their weights typically used in refinement may, need revision. A detailed comparison of the six structures has provided, experimental evidence on the role of Lys41 in catalysis. Furthermore, insights are given into the structural effects related to the pH-dependent, binding of a sulfate anion, which mimics the phosphate group of RNA, in, the active site. Finally, the results support a number of thermodynamic, and kinetic experimental data concerning the role of the disulfide bridge, between Cys65 and Cys72 in the folding of RNase A.

About this Structure

1KF8 is a Single protein structure of sequence from Bos taurus. Active as Pancreatic ribonuclease, with EC number 3.1.27.5 Full crystallographic information is available from OCA.

Reference

Atomic resolution structures of ribonuclease A at six pH values., Berisio R, Sica F, Lamzin VS, Wilson KS, Zagari A, Mazzarella L, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):441-50. Epub 2002, Feb 21. PMID:11856829

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