1x60

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{{STRUCTURE_1x60| PDB=1x60 | SCENE= }}
{{STRUCTURE_1x60| PDB=1x60 | SCENE= }}
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'''Solution structure of the peptidoglycan binding domain of B. subtilis cell wall lytic enzyme CwlC'''
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===Solution structure of the peptidoglycan binding domain of B. subtilis cell wall lytic enzyme CwlC===
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==Overview==
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Bacillus subtilis CwlC is a cell wall lytic N-acetylmuramoyl-l-alanine amidase that plays an important role in mother-cell lysis during sporulation. The enzyme consists of an N-terminal catalytic domain with C-terminal tandem repeats. The repeats [repeat 1 (residues 184-219) and repeat 2 (residues 220-255)] are termed CwlCr. We report on the solution structure of CwlCr as determined by multidimensional NMR, including the use of 36 (h3)J(NC)'-derived hydrogen bond restraints and 64 residual (1)D(NH) dipolar couplings. Two tandem repeats fold into a pseudo-2-fold symmetric single-domain structure consisting of a betaalphabetabetaalphabeta-fold containing numerous contacts between the repeats. Hydrophobic residues important for structural integrity are conserved between the repeats, and are located symmetrically. We also present NMR analysis of the circularly permuted repeat mutant of CwlCr. Secondary structure content from the chemical shifts and hydrogen bonds derived from (h3)J(NC)' show that the mutant folds into a structure similar to that of the wild type, suggesting that the repeats are exchangeable. This implies that conserved hydrophobic residues are crucial for maintaining the folding of the repeats. While monitoring the chemical shift perturbations following the addition of digested soluble peptidoglycan fragments, we identified two peptidoglycan interaction sites of CwlCr at the edges of the protein symmetrically, and they are located approximately 28 A from each other.
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(as it appears on PubMed at http://www.pubmed.gov), where 16042392 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16042392}}
==About this Structure==
==About this Structure==
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1X60 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X60 OCA].
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1X60 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X60 OCA].
==Reference==
==Reference==
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[[Category: Peptidoglycan]]
[[Category: Peptidoglycan]]
[[Category: Tandem repeat]]
[[Category: Tandem repeat]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:36:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:05:17 2008''

Revision as of 14:05, 27 July 2008

Image:1x60.png

Template:STRUCTURE 1x60

Solution structure of the peptidoglycan binding domain of B. subtilis cell wall lytic enzyme CwlC

Template:ABSTRACT PUBMED 16042392

About this Structure

1X60 is a Single protein structure of sequence from Bacillus subtilis. Full experimental information is available from OCA.

Reference

Solution structure of the peptidoglycan binding domain of Bacillus subtilis cell wall lytic enzyme CwlC: characterization of the sporulation-related repeats by NMR., Mishima M, Shida T, Yabuki K, Kato K, Sekiguchi J, Kojima C, Biochemistry. 2005 Aug 2;44(30):10153-63. PMID:16042392

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