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1ohz

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[[Image:1ohz.gif|left|200px]]
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{{STRUCTURE_1ohz| PDB=1ohz | SCENE= }}
{{STRUCTURE_1ohz| PDB=1ohz | SCENE= }}
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'''COHESIN-DOCKERIN COMPLEX FROM THE CELLULOSOME OF CLOSTRIDIUM THERMOCELLUM'''
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===COHESIN-DOCKERIN COMPLEX FROM THE CELLULOSOME OF CLOSTRIDIUM THERMOCELLUM===
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==Overview==
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The utilization of organized supramolecular assemblies to exploit the synergistic interactions afforded by close proximity, both for enzymatic synthesis and for the degradation of recalcitrant substrates, is an emerging theme in cellular biology. Anaerobic bacteria harness a multiprotein complex, termed the "cellulosome," for efficient degradation of the plant cell wall. This megadalton catalytic machine organizes an enzymatic consortium on a multifaceted molecular scaffold whose "cohesin" domains interact with corresponding "dockerin" domains of the enzymes. Here we report the structure of the cohesin-dockerin complex from Clostridium thermocellum at 2.2-A resolution. The data show that the beta-sheet cohesin domain interacts predominantly with one of the helices of the dockerin. Whereas the structure of the cohesin remains essentially unchanged, the loop-helix-helix-loop-helix motif of the dockerin undergoes conformational change and ordering compared with its solution structure, although the classical 12-residue EF-hand coordination to two calcium ions is maintained. Significantly, internal sequence duplication within the dockerin is manifested in near-perfect internal twofold symmetry, suggesting that both "halves" of the dockerin may interact with cohesins in a similar manner, thus providing a higher level of structure to the cellulosome and possibly explaining the presence of "polycellulosomes." The structure provides an explanation for the lack of cross-species recognition between cohesin-dockerin pairs and thus provides a blueprint for the rational design, construction, and exploitation of these catalytic assemblies.
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(as it appears on PubMed at http://www.pubmed.gov), where 14623971 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14623971}}
==About this Structure==
==About this Structure==
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[[Category: Cohesin]]
[[Category: Cohesin]]
[[Category: Dockerin]]
[[Category: Dockerin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:52:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:11:42 2008''

Revision as of 14:11, 27 July 2008

Image:1ohz.png

Template:STRUCTURE 1ohz

COHESIN-DOCKERIN COMPLEX FROM THE CELLULOSOME OF CLOSTRIDIUM THERMOCELLUM

Template:ABSTRACT PUBMED 14623971

About this Structure

1OHZ is a Protein complex structure of sequences from Clostridium thermocellum. Full crystallographic information is available from OCA.

Reference

Cellulosome assembly revealed by the crystal structure of the cohesin-dockerin complex., Carvalho AL, Dias FM, Prates JA, Nagy T, Gilbert HJ, Davies GJ, Ferreira LM, Romao MJ, Fontes CM, Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):13809-14. Epub 2003 Nov 17. PMID:14623971

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