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1orx

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{{STRUCTURE_1orx| PDB=1orx | SCENE= }}
{{STRUCTURE_1orx| PDB=1orx | SCENE= }}
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'''Solution Structure of the acyclic permutant des-(24-28)-kalata B1.'''
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===Solution Structure of the acyclic permutant des-(24-28)-kalata B1.===
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==Overview==
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Cyclotides are a recently discovered family of disulfide rich proteins from plants that contain a circular protein backbone. They are exceptionally stable, as exemplified by their use in native medicine of the prototypic cyclotide kalata B1. The peptide retains uterotonic activity after the plant from which it is derived is boiled to make a medicinal tea. The circular backbone is thought to be in part responsible for the stability of the cyclotides, and to investigate its role in determining structure and biological activity, an acyclic derivative, des-(24-28)-kalata B1, was chemically synthesized and purified. This derivative has five residues removed from the 29-amino acid circular backbone of kalata B1 in a loop region corresponding to a processing site in the biosynthetic precursor protein. Two-dimensional NMR spectra of the peptide were recorded, assigned, and used to identify a series of distance, angle, and hydrogen bonding restraints. These were in turn used to determine a representative family of solution structures. Of particular interest was a determination of the structural similarities and differences between des-(24-28)-kalata B1 and native kalata B1. Although the overall three-dimensional fold remains very similar to that of the native circular protein, removal of residues 24-28 of kalata B1 causes disruption of some structural features that are important to the overall stability. Furthermore, loss of hemolytic activity is associated with backbone truncation and linearization.
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(as it appears on PubMed at http://www.pubmed.gov), where 12779323 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12779323}}
==About this Structure==
==About this Structure==
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1ORX is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ORX OCA].
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1ORX is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ORX OCA].
==Reference==
==Reference==
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[[Category: Cyclotide]]
[[Category: Cyclotide]]
[[Category: Kalata b1]]
[[Category: Kalata b1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:12:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:17:24 2008''

Revision as of 14:17, 27 July 2008

Image:1orx.png

Template:STRUCTURE 1orx

Solution Structure of the acyclic permutant des-(24-28)-kalata B1.

Template:ABSTRACT PUBMED 12779323

About this Structure

1ORX is a Single protein structure. Full experimental information is available from OCA.

Reference

Linearization of a naturally occurring circular protein maintains structure but eliminates hemolytic activity., Barry DG, Daly NL, Clark RJ, Sando L, Craik DJ, Biochemistry. 2003 Jun 10;42(22):6688-95. PMID:12779323

Page seeded by OCA on Sun Jul 27 17:17:24 2008

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