This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2auv

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2auv.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2auv.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2auv| PDB=2auv | SCENE= }}
{{STRUCTURE_2auv| PDB=2auv | SCENE= }}
-
'''Solution Structure of HndAc : A Thioredoxin-like [2Fe-2S] Ferredoxin Involved in the NADP-reducing Hydrogenase Complex'''
+
===Solution Structure of HndAc : A Thioredoxin-like [2Fe-2S] Ferredoxin Involved in the NADP-reducing Hydrogenase Complex===
-
==Overview==
+
<!--
-
The NADP-reducing hydrogenase complex from Desulfovibrio fructosovorans is a heterotetramer encoded by the hndABCD operon. Sequence analysis indicates that the HndC subunit (52 kDa) corresponds to the NADP-reducing unit, and the HndD subunit (63.5 kDa) is homologous to Clostridium pasteurianum hydrogenase. The role of HndA and HndB subunits (18.8 kDa and 13.8 kDa, respectively) in the complex remains unknown. The HndA subunit belongs to the [2Fe-2S] ferredoxin family typified by C. pasteurianum ferredoxin. HndA is organized into two independent structural domains, and we report in the present work the NMR structure of its C-terminal domain, HndAc. HndAc has a thioredoxin-like fold consisting in four beta-strands and two relatively long helices. The [2Fe-2S] cluster is located near the surface of the protein and bound to four cysteine residues particularly well conserved in this class of proteins. Electron exchange between the HndD N-terminal [2Fe-2S] domain (HndDN) and HndAc has been previously evidenced, and in the present studies we have mapped the binding site of the HndDN domain on HndAc. A structural analysis of HndB indicates that it is a FeS subunit with 41% similarity with HndAc and it contains a possible thioredoxin-like fold. Our data let us propose that HndAc and HndB can form a heterodimeric intermediate in the electron transfer between the hydrogenase (HndD) active site and the NADP reduction site in HndC.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16731971}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16731971 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16731971}}
==About this Structure==
==About this Structure==
-
2AUV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_fructosovorans Desulfovibrio fructosovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUV OCA].
+
2AUV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_fructosovorans Desulfovibrio fructosovorans]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUV OCA].
==Reference==
==Reference==
Line 30: Line 34:
[[Category: Thiordoxin-like]]
[[Category: Thiordoxin-like]]
[[Category: Thioredoxin]]
[[Category: Thioredoxin]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:30:07 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:20:35 2008''

Revision as of 14:20, 27 July 2008

Image:2auv.png

Template:STRUCTURE 2auv

Solution Structure of HndAc : A Thioredoxin-like [2Fe-2S] Ferredoxin Involved in the NADP-reducing Hydrogenase Complex

Template:ABSTRACT PUBMED 16731971

About this Structure

2AUV is a Single protein structure of sequence from Desulfovibrio fructosovorans. Full experimental information is available from OCA.

Reference

Solution structure of HndAc: a thioredoxin-like domain involved in the NADP-reducing hydrogenase complex., Nouailler M, Morelli X, Bornet O, Chetrit B, Dermoun Z, Guerlesquin F, Protein Sci. 2006 Jun;15(6):1369-78. PMID:16731971

Page seeded by OCA on Sun Jul 27 17:20:35 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2auv"
Personal tools