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1uvh

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[[Image:1uvh.gif|left|200px]]
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{{STRUCTURE_1uvh| PDB=1uvh | SCENE= }}
{{STRUCTURE_1uvh| PDB=1uvh | SCENE= }}
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'''X-RAY STRUCTURE OF DPS FROM MYCOBACTERIUM SMEGMATIS'''
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===X-RAY STRUCTURE OF DPS FROM MYCOBACTERIUM SMEGMATIS===
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==Overview==
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The structure and function of Mycobacterium smegmatis Dps (DNA-binding proteins from starved cells) and of the protein studied by Gupta and Chatterji, in which the C terminus that is used for binding DNA contains a histidine tag, have been characterized in parallel. The native dodecamer dissociated reversibly into dimers above pH 7.5 and below pH 6.0, with apparent pK(a) values of approximately 7.65 and 4.75; at pH approximately 4.0, dimers formed monomers. Based on structural analysis, the two dissociation steps have been attributed to breakage of the salt bridges between Glu(157) and Arg(99) located at the 3-fold symmetry axes and to protonation of Asp(66) hydrogen-bonded to Lys(36) across the dimer interface, respectively. The C-terminal tag did not affect subunit dissociation, but altered DNA binding dramatically. At neutral pH, protonation of the histidine tag promoted DNA condensation, whereas in the native C terminus, compensation of negative and positive charges led to DNA binding without condensation. This different mode of interaction with DNA has important functional consequences as indicated by the failure of the native protein to protect DNA from DNase-mediated cleavage and by the efficiency of the tagged protein in doing so as a result of DNA sequestration in the condensates. Chemical protection of DNA from oxidative damage is realized by Dps proteins in a multistep iron oxidation/uptake/mineralization process. Dimers have a decreased protection efficiency due to disruption of the dodecamer internal cavity, where iron is deposited and mineralized after oxidation at the ferroxidase center.
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{{ABSTRACT_PUBMED_16030020}}
==About this Structure==
==About this Structure==
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[[Category: Dna binding protein]]
[[Category: Dna binding protein]]
[[Category: Dna protection from oxidative damage]]
[[Category: Dna protection from oxidative damage]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:24:10 2008''

Revision as of 14:24, 27 July 2008

Image:1uvh.png

Template:STRUCTURE 1uvh

X-RAY STRUCTURE OF DPS FROM MYCOBACTERIUM SMEGMATIS

Template:ABSTRACT PUBMED 16030020

About this Structure

1UVH is a Single protein structure of sequence from Mycobacterium smegmatis. Full crystallographic information is available from OCA.

Reference

Reassessment of protein stability, DNA binding, and protection of Mycobacterium smegmatis Dps., Ceci P, Ilari A, Falvo E, Giangiacomo L, Chiancone E, J Biol Chem. 2005 Oct 14;280(41):34776-85. Epub 2005 Jul 19. PMID:16030020

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