This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1khq
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1khq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1khq, resolution 1.6Å" /> '''ORTHORHOMBIC FORM OF ...)
Next diff →
Revision as of 17:07, 20 November 2007
|
ORTHORHOMBIC FORM OF PAPAIN/ZLFG-DAM COVALENT COMPLEX
Overview
The three-dimensional structure of two polymorphs of a ZLFG-CH2-papain, covalent complex has been determined by X-ray crystallography. The, structures indicate that: (i) the methylene carbon atom of the inhibitor, is covalently bound to the Sgamma atom of Cys25 of papain; (ii) the, hydrophobic S2 pocket formed by Pro68, Val133, Val157, and Asp158 is, occupied by the inhibitor's phenylalanyl P2 side chain; (iii) extensive, hydrogen bonding and hydrophobic interactions are responsible for the, interaction of the inhibitor with the enzyme. Comparison with similar, structures suggests that in covalent complexes preservation of main, chain-main chain interactions between the enzyme and the inhibitor may, have higher priority than the P-S interactions.
About this Structure
1KHQ is a Single protein structure of sequence from Carica papaya. Active as Papain, with EC number 3.4.22.2 Full crystallographic information is available from OCA.
Reference
Two polymorphs of a covalent complex between papain and a diazomethylketone inhibitor., Janowski R, Kozak M, Jankowska E, Grzonka Z, Jaskolski M, J Pept Res. 2004 Oct;64(4):141-50. PMID:15357669
Page seeded by OCA on Tue Nov 20 19:14:50 2007
