2o4u

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{{STRUCTURE_2o4u| PDB=2o4u | SCENE= }}
{{STRUCTURE_2o4u| PDB=2o4u | SCENE= }}
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'''Crystal structure of Mammalian Dimeric Dihydrodiol Dehydrogenase'''
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===Crystal structure of Mammalian Dimeric Dihydrodiol Dehydrogenase===
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==Overview==
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Dimeric dihydrodiol dehydrogenase (DD) catalyses the nicotinamide adenine dinucleotide phosphate (NADP+)-dependent oxidation of trans-dihydrodiols of aromatic hydrocarbons to their corresponding catechols. This is the first report of the crystal structure of the dimeric enzyme determined at 2.0 A resolution. The tertiary structure is formed by a classical dinucleotide binding fold comprising of two betaalphabetaalphabeta motifs at the N-terminus and an eight-stranded, predominantly antiparallel beta-sheet at the C-terminus. The active-site of DD, occupied either by a glycerol molecule or the inhibitor 4-hydroxyacetophenone, is located in the C-terminal domain of the protein and maintained by a number of residues including Lys97, Trp125, Phe154, Leu158, Val161, Asp176, Leu177, Tyr180, Trp254, Phe279, and Asp280. The dimer interface is stabilized by a large number of intermolecular contacts mediated by the beta-sheet of each monomer, which includes an intricate hydrogen bonding network maintained in principal by Arg148 and Arg202. Site-directed mutagenesis has demonstrated that the intact dimer is not essential for catalytic activity. The similarity between the quaternary structures of mammalian DD and glucose-fructose oxidoreductase isolated from the prokaryotic organism Zymomonas mobilis suggests that both enzymes are members of a unique family of oligomeric proteins and may share a common ancestral gene.
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{{ABSTRACT_PUBMED_17654552}}
==About this Structure==
==About this Structure==
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[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Predominantly anti-parallel beta sheet]]
[[Category: Predominantly anti-parallel beta sheet]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:19:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:28:54 2008''

Revision as of 14:28, 27 July 2008

Image:2o4u.png

Template:STRUCTURE 2o4u

Crystal structure of Mammalian Dimeric Dihydrodiol Dehydrogenase

Template:ABSTRACT PUBMED 17654552

About this Structure

2O4U is a Single protein structure of sequence from Macaca fascicularis. Full crystallographic information is available from OCA.

Reference

Structures of dimeric dihydrodiol dehydrogenase apoenzyme and inhibitor complex: probing the subunit interface with site-directed mutagenesis., Carbone V, Endo S, Sumii R, Chung RP, Matsunaga T, Hara A, El-Kabbani O, Proteins. 2008 Jan 1;70(1):176-87. PMID:17654552

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