1yt6

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[[Image:1yt6.gif|left|200px]]
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{{STRUCTURE_1yt6| PDB=1yt6 | SCENE= }}
{{STRUCTURE_1yt6| PDB=1yt6 | SCENE= }}
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'''NMR structure of peptide SD'''
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===NMR structure of peptide SD===
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==Overview==
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Using NMR spectroscopy and simulated annealing calculations, we determined the solution structure of the disulfide-linked cyclized decapeptide ACLPWSDGPC (SD), which is bound to an anti-(gibberellin A(4)) mAb 4-B8(8)/E9 and was found to be the first peptidyl mimotope for a hydrophobic ligand. The resulting structure of the peptide showed a beta-turn-like conformation in residues three to seven and the region converges well (average rmsd 0.54 A). The binding activity and the epitopes of the peptide to the antibody were assessed using saturation transfer difference (STD)-NMR experiments. We also conducted docking simulations between the peptide and the mAb to determine how the peptide is bound to the mAb. Resonances around the beta-turn-like conformation of peptide SD (residues 3-5) showed strong STD enhancement, which agreed well with results from docking simulation between peptide SD and the mAb. Together with the commonality of amino acid residues of the mAb involved in interactions with gibberellin A(4) (GA(4)) and peptide SD, we concluded that peptide SD is bound to the antigen-binding site of mAb 4-B8(8)/E9 as a GA(4) mimic, confirming evidence for the existence of peptide mimics even for hydrophobic ligands.
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The line below this paragraph, {{ABSTRACT_PUBMED_16176267}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16176267 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16176267}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YT6 OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YT6 OCA].
==Reference==
==Reference==
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[[Category: Mimic]]
[[Category: Mimic]]
[[Category: Std-nmr]]
[[Category: Std-nmr]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:45:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:32:38 2008''

Revision as of 14:32, 27 July 2008

Image:1yt6.png

Template:STRUCTURE 1yt6

NMR structure of peptide SD

Template:ABSTRACT PUBMED 16176267

About this Structure

Full experimental information is available from OCA.

Reference

Structure, epitope mapping, and docking simulation of a gibberellin mimic peptide as a peptidyl mimotope for a hydrophobic ligand., Murata T, Hemmi H, Nakamura S, Shimizu K, Suzuki Y, Yamaguchi I, FEBS J. 2005 Oct;272(19):4938-48. PMID:16176267

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