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1zm1

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[[Image:1zm1.gif|left|200px]]
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{{STRUCTURE_1zm1| PDB=1zm1 | SCENE= }}
{{STRUCTURE_1zm1| PDB=1zm1 | SCENE= }}
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'''Crystal structures of complex F. succinogenes 1,3-1,4-beta-D-glucanase and beta-1,3-1,4-cellotriose'''
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===Crystal structures of complex F. succinogenes 1,3-1,4-beta-D-glucanase and beta-1,3-1,4-cellotriose===
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==Overview==
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Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase (Fsbeta-glucanase) catalyzes the specific hydrolysis of beta-1,4 glycosidic bonds adjacent to beta-1,3 linkages in beta-D-glucans or lichenan. This is the first report to elucidate the crystal structure of a truncated Fsbeta-glucanase (TFsbeta-glucanase) in complex with beta-1,3-1,4-cellotriose, a major product of the enzyme reaction. The crystal structures, at a resolution of 2.3 angstroms, reveal that the overall fold of TFsbeta-glucanase remains virtually unchanged upon sugar binding. The enzyme accommodates five glucose residues, forming a concave active cleft. The beta-1,3-1,4-cellotriose with subsites -3 to -1 bound to the active cleft of TFsbeta-glucanase with its reducing end subsite -1 close to the key catalytic residues Glu56 and Glu60. All three subsites of the beta-1,3-1,4-cellotriose adopted a relaxed C(1)4 conformation, with a beta-1,3 glycosidic linkage between subsites -2 and -1, and a beta-1,4 glycosidic linkage between subsites -3 and -2. On the basis of the enzyme-product complex structure observed in this study, a catalytic mechanism and substrate binding conformation of the active site of TFsbeta-glucanase is proposed.
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The line below this paragraph, {{ABSTRACT_PUBMED_16246371}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16246371 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16246371}}
==About this Structure==
==About this Structure==
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[[Category: Active cleft]]
[[Category: Active cleft]]
[[Category: Glucanase/1,3-1,4-beta-d-glucanase]]
[[Category: Glucanase/1,3-1,4-beta-d-glucanase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:47:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:34:38 2008''

Revision as of 14:34, 27 July 2008

Image:1zm1.png

Template:STRUCTURE 1zm1

Crystal structures of complex F. succinogenes 1,3-1,4-beta-D-glucanase and beta-1,3-1,4-cellotriose

Template:ABSTRACT PUBMED 16246371

About this Structure

1ZM1 is a Single protein structure of sequence from Fibrobacter succinogenes. Full crystallographic information is available from OCA.

Reference

Crystal structure of truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase in complex with beta-1,3-1,4-cellotriose., Tsai LC, Shyur LF, Cheng YS, Lee SH, J Mol Biol. 2005 Dec 2;354(3):642-51. Epub 2005 Sep 30. PMID:16246371

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