1te6

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{{STRUCTURE_1te6| PDB=1te6 | SCENE= }}
{{STRUCTURE_1te6| PDB=1te6 | SCENE= }}
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'''Crystal Structure of Human Neuron Specific Enolase at 1.8 angstrom'''
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===Crystal Structure of Human Neuron Specific Enolase at 1.8 angstrom===
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==Overview==
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Human neuron-specific enolase (NSE) or isozyme gamma has been expressed with a C-terminal His-tag in Escherichia coli. The enzyme has been purified, crystallized and its crystal structure determined. In the crystals the enzyme forms the asymmetric complex NSE x Mg2 x SO4/NSE x Mg x Cl, where "/" separates the dimer subunits. The subunit that contains the sulfate (or phosphate) ion and two magnesium ions is in the closed conformation observed in enolase complexes with the substrate or its analogues; the other subunit is in the open conformation observed in enolase subunits without bound substrate or analogues. This indicates negative cooperativity for ligand binding between subunits. Electrostatic charge differences between isozymes alpha and gamma, -19 at physiological pH, are concentrated in the regions of the molecular surface that are negatively charged in alpha, i.e. surface areas negatively charged in alpha are more negatively charged in gamma, while areas that are neutral or positively charged tend to be charge-conserved.
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{{ABSTRACT_PUBMED_15289101}}
==About this Structure==
==About this Structure==
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[[Category: Neuron]]
[[Category: Neuron]]
[[Category: Surface charge]]
[[Category: Surface charge]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:50:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:40:06 2008''

Revision as of 14:40, 27 July 2008

Image:1te6.png

Template:STRUCTURE 1te6

Crystal Structure of Human Neuron Specific Enolase at 1.8 angstrom

Template:ABSTRACT PUBMED 15289101

About this Structure

1TE6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Expression, purification and the 1.8 angstroms resolution crystal structure of human neuron specific enolase., Chai G, Brewer JM, Lovelace LL, Aoki T, Minor W, Lebioda L, J Mol Biol. 2004 Aug 20;341(4):1015-21. PMID:15289101

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