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1qdf

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{{STRUCTURE_1qdf| PDB=1qdf | SCENE= }}
{{STRUCTURE_1qdf| PDB=1qdf | SCENE= }}
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'''THE NMR STUDY OF DNA QUADRUPLEX STRUCTURE, APTAMER (15MER) DNA'''
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===THE NMR STUDY OF DNA QUADRUPLEX STRUCTURE, APTAMER (15MER) DNA===
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==Overview==
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The interaction of a DNA quadruplex with thrombin has been studied by first determining the sites of manganese binding to the quadruplex in the absence of thrombin. This has been followed by determining if the interactions with thrombin displace the bound manganese. A different DNA quadruplex has also been studied as a control. The refined solution structures of two DNA quadruplexes have been used to predict the electrostatic potentials of these DNAs. The calculated electrostatic potentials have been used to predict the locations of the binding sites of the paramagnetic ion manganese to these DNAs. The enhanced relaxation of DNA protons due to the binding of the paramagnetic metal ion Mn2+ has been used to experimentally determine the locations of the binding sites. The NMR results and the predictions based on the electrostatic potentials both place the binding sites of the manganese in the narrow grooves of these quadruplex DNAs. The predicted locations are spatially close to those experimentally observed, and the predicted and experimental locations also have similar electrostatic potential energy. These results have allowed a validation of the predictions of electrostatic potentials from structure. The 15mer quadruplex has two strong Mn2+ binding sites with one in each narrow groove. Both Mn2+ are released when the 15mer is complexed with thrombin, indicating that both narrow grooves are involved in the 15mer-thrombin interactions. The dimer quadruplex has a different structural motif than the 15mer and the presence of thrombin does not appreciably affect its interactions with Mn2+.
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(as it appears on PubMed at http://www.pubmed.gov), where 8757801 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8757801}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QDF OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QDF OCA].
==Reference==
==Reference==
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[[Category: Telomere dna]]
[[Category: Telomere dna]]
[[Category: Thrombin inhibitor]]
[[Category: Thrombin inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:09:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:45:27 2008''

Revision as of 14:45, 27 July 2008

Image:1qdf.png

Template:STRUCTURE 1qdf

THE NMR STUDY OF DNA QUADRUPLEX STRUCTURE, APTAMER (15MER) DNA

Template:ABSTRACT PUBMED 8757801

About this Structure

Full experimental information is available from OCA.

Reference

Determination of the number and location of the manganese binding sites of DNA quadruplexes in solution by EPR and NMR in the presence and absence of thrombin., Marathias VM, Wang KY, Kumar S, Pham TQ, Swaminathan S, Bolton PH, J Mol Biol. 1996 Jul 19;260(3):378-94. PMID:8757801

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