2gj9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2gj9.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2gj9.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2gj9| PDB=2gj9 | SCENE= }}
{{STRUCTURE_2gj9| PDB=2gj9 | SCENE= }}
-
'''Structure of the MnmE G-domain in complex with GDP*AlF4-, Mg2+ and Rb+'''
+
===Structure of the MnmE G-domain in complex with GDP*AlF4-, Mg2+ and Rb+===
-
==Overview==
+
<!--
-
MnmE, a Guanine nucleotide-binding protein conserved between bacteria and man, is involved in the modification of tRNAs. Here we provide biochemical and X-ray structural evidence for a new GTP-hydrolysis mechanism, where the G-domains of MnmE dimerise in a potassium-dependent manner and induce GTP hydrolysis. The structure in the presence of GDP-AlFx and potassium shows how juxtaposition of the subunits induces a conformational change around the nucleotide which reorients the catalytic machinery. A critical glutamate is positioned such as to stabilise or activate the attacking water. Potassium provides a positive charge into the catalytic site in a position analogous to the arginine finger in the Ras-RasGAP system. Mutational studies show that potassium-dependent dimerisation and GTP hydrolysis can be uncoupled and that interaction between the G-domains is a prerequisite for subsequent phosphoryl transfer. We propose a model for the juxtaposition of G-domains in the full-length protein and how it induces conformational changes in the putative tRNA-modification centre.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16763562}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16763562 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16763562}}
==About this Structure==
==About this Structure==
Line 26: Line 30:
[[Category: Alpha-beta-sandwich]]
[[Category: Alpha-beta-sandwich]]
[[Category: G-domain dimer]]
[[Category: G-domain dimer]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:10:15 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:48:19 2008''

Revision as of 14:48, 27 July 2008

Image:2gj9.png

Template:STRUCTURE 2gj9

Structure of the MnmE G-domain in complex with GDP*AlF4-, Mg2+ and Rb+

Template:ABSTRACT PUBMED 16763562

About this Structure

2GJ9 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Dimerisation-dependent GTPase reaction of MnmE: how potassium acts as GTPase-activating element., Scrima A, Wittinghofer A, EMBO J. 2006 Jun 21;25(12):2940-51. Epub 2006 Jun 8. PMID:16763562

Page seeded by OCA on Sun Jul 27 17:48:19 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gj9"
Personal tools