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2r70

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{{STRUCTURE_2r70| PDB=2r70 | SCENE= }}
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'''Crystal structure of infectious bursal disease virus VP1 polymerase, cocrystallized with an oligopeptide mimicking the VP3 C-terminus.'''
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===Crystal structure of infectious bursal disease virus VP1 polymerase, cocrystallized with an oligopeptide mimicking the VP3 C-terminus.===
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==Overview==
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Two lineages of viral RNA-dependent RNA polymerases (RDRPs) differing in the organization (canonical vs. noncanonical) of the palm subdomain have been identified. Phylogenetic analyses indicate that both lineages diverged at a very early stage of the evolution of the enzyme [Gorbalenya AE, Pringle FM, Zeddam JL, Luke BT, Cameron CE, Kalmakoff J, Hanzlik TN, Gordon KH, Ward VK (2002) J Mol Biol 324:47-62]. Here, we report the x-ray structure of a noncanonical birnaviral RDRP, named VP1, in its free form, bound to Mg(2+) ions, and bound to a peptide representing the polymerase-binding motif of the regulatory viral protein VP3. The structure of VP1 reveals that the noncanonical connectivity of the palm subdomain maintains the geometry of the catalytic residues found in canonical polymerases but results in a partial blocking of the active site cavity. The VP1-VP3 peptide complex shows a mode of polymerase activation in which VP3 binding promotes a conformational change that removes the steric blockade of the VP1 active site, facilitating the accommodation of the template and incoming nucleotides for catalysis. The striking structural similarities between birnavirus (dsRNA) and the positive-stranded RNA picornavirus and calicivirus RDRPs provide evidence supporting the existence of functional and evolutionary relationships between these two virus groups.
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(as it appears on PubMed at http://www.pubmed.gov), where 18077388 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18077388}}
==About this Structure==
==About this Structure==
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[[Category: Vp1]]
[[Category: Vp1]]
[[Category: Vp3]]
[[Category: Vp3]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:21:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:50:12 2008''

Revision as of 14:50, 27 July 2008

Image:2r70.png

Template:STRUCTURE 2r70

Crystal structure of infectious bursal disease virus VP1 polymerase, cocrystallized with an oligopeptide mimicking the VP3 C-terminus.

Template:ABSTRACT PUBMED 18077388

About this Structure

Full crystallographic information is available from OCA.

Reference

Activation mechanism of a noncanonical RNA-dependent RNA polymerase., Garriga D, Navarro A, Querol-Audi J, Abaitua F, Rodriguez JF, Verdaguer N, Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20540-5. Epub 2007 Dec 11. PMID:18077388

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