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1qfl

From Proteopedia

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{{STRUCTURE_1qfl| PDB=1qfl | SCENE= }}
{{STRUCTURE_1qfl| PDB=1qfl | SCENE= }}
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'''BIOSYNTHETIC THIOLASE FROM ZOOGLOEA RAMIGERA IN COMPLEX WITH A REACTION INTERMEDIATE.'''
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===BIOSYNTHETIC THIOLASE FROM ZOOGLOEA RAMIGERA IN COMPLEX WITH A REACTION INTERMEDIATE.===
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==Overview==
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BACKGROUND: Thiolases are ubiquitous and form a large family of dimeric or tetrameric enzymes with a conserved, five-layered alphabetaalphabetaalpha catalytic domain. Thiolases can function either degradatively, in the beta-oxidation pathway of fatty acids, or biosynthetically. Biosynthetic thiolases catalyze the biological Claisen condensation of two molecules of acetyl-CoA to form acetoacetyl-CoA. This is one of the fundamental categories of carbon skeletal assembly patterns in biological systems and is the first step in a wide range of biosynthetic pathways, including those that generate cholesterol, steroid hormones, and various energy-storage molecules. RESULTS: The crystal structure of the tetrameric biosynthetic thiolase from Zoogloea ramigera has been determined at 2.0 A resolution. The structure contains a striking and novel 'cage-like' tetramerization motif, which allows for some hinge motion of the two tight dimers with respect to each other. The protein crystals were flash-frozen after a short soak with the enzyme's substrate, acetoacetyl-CoA. A reaction intermediate was thus trapped: the enzyme tetramer is acetylated at Cys89 and has a CoA molecule bound in each of its active-site pockets. CONCLUSIONS: The shape of the substrate-binding pocket reveals the basis for the short-chain substrate specificity of the enzyme. The active-site architecture, and in particular the position of the covalently attached acetyl group, allow a more detailed reaction mechanism to be proposed in which Cys378 is involved in both steps of the reaction. The structure also suggests an important role for the thioester oxygen atom of the acetylated enzyme in catalysis.
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(as it appears on PubMed at http://www.pubmed.gov), where 10545327 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10545327}}
==About this Structure==
==About this Structure==
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[[Category: Tetramerization motif]]
[[Category: Tetramerization motif]]
[[Category: Thiolase]]
[[Category: Thiolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:12:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:50:13 2008''

Revision as of 14:50, 27 July 2008

Image:1qfl.png

Template:STRUCTURE 1qfl

BIOSYNTHETIC THIOLASE FROM ZOOGLOEA RAMIGERA IN COMPLEX WITH A REACTION INTERMEDIATE.

Template:ABSTRACT PUBMED 10545327

About this Structure

1QFL is a Single protein structure of sequence from Zoogloea ramigera. Full crystallographic information is available from OCA.

Reference

A biosynthetic thiolase in complex with a reaction intermediate: the crystal structure provides new insights into the catalytic mechanism., Modis Y, Wierenga RK, Structure. 1999 Oct 15;7(10):1279-90. PMID:10545327

Page seeded by OCA on Sun Jul 27 17:50:13 2008

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