1mwj

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[[Image:1mwj.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1mwj| PDB=1mwj | SCENE= }}
{{STRUCTURE_1mwj| PDB=1mwj | SCENE= }}
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'''Crystal Structure of a MUG-DNA pseudo substrate complex'''
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===Crystal Structure of a MUG-DNA pseudo substrate complex===
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==Overview==
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The bacterial mismatch-specific uracil-DNA glycosylase (MUG) and eukaryotic thymine-DNA glycosylase (TDG) enzymes form a homologous family of DNA glycosylases that initiate base-excision repair of G:U/T mismatches. Despite low sequence homology, the MUG/TDG enzymes are structurally related to the uracil-DNA glycosylase enzymes, but have a very different mechanism for substrate recognition. We have now determined the crystal structure of the Escherichia coli MUG enzyme complexed with an oligonucleotide containing a non-hydrolysable deoxyuridine analogue mismatched with guanine, providing the first structure of an intact substrate-nucleotide productively bound to a hydrolytic DNA glycosylase. The structure of this complex explains the preference for G:U over G:T mispairs, and reveals an essentially non-specific pyrimidine-binding pocket that allows MUG/TDG enzymes to excise the alkylated base, 3, N(4)-ethenocytosine. Together with structures for the free enzyme and for an abasic-DNA product complex, the MUG-substrate analogue complex reveals the conformational changes accompanying the catalytic cycle of substrate binding, base excision and product release.
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The line below this paragraph, {{ABSTRACT_PUBMED_10581234}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 10581234 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10581234}}
==About this Structure==
==About this Structure==
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[[Category: Rossmann fold]]
[[Category: Rossmann fold]]
[[Category: Uracil recognition.]]
[[Category: Uracil recognition.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:48:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:51:45 2008''

Revision as of 14:51, 27 July 2008

Image:1mwj.png

Template:STRUCTURE 1mwj

Crystal Structure of a MUG-DNA pseudo substrate complex

Template:ABSTRACT PUBMED 10581234

About this Structure

1MWJ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of a thwarted mismatch glycosylase DNA repair complex., Barrett TE, Scharer OD, Savva R, Brown T, Jiricny J, Verdine GL, Pearl LH, EMBO J. 1999 Dec 1;18(23):6599-609. PMID:10581234

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