2flp

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[[Image:2flp.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_2flp| PDB=2flp | SCENE= }}
{{STRUCTURE_2flp| PDB=2flp | SCENE= }}
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'''Binary complex of the catalytic core of human DNA polymerase iota with DNA (template G)'''
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===Binary complex of the catalytic core of human DNA polymerase iota with DNA (template G)===
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==Overview==
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Substrate-induced conformational change of the protein is the linchpin of enzymatic reactions. Replicative DNA polymerases, for example, convert from an open to a closed conformation in response to dNTP binding. Human DNA polymerase-iota (hPoliota), a member of the Y family of DNA polymerases, differs strikingly from other polymerases in its much higher proficiency and fidelity for nucleotide incorporation opposite template purines than opposite template pyrimidines. We present here a crystallographic analysis of hPoliota binary complexes, which together with the ternary complexes show that, contrary to replicative DNA polymerases, the DNA, and not the polymerase, undergoes the primary substrate-induced conformational change. The incoming dNTP "pushes" templates A and G from the anti to the syn conformation dictated by a rigid hPoliota active site. Together, the structures posit a mechanism for template selection wherein dNTP binding induces a conformational switch in template purines for productive Hoogsteen base pairing.
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The line below this paragraph, {{ABSTRACT_PUBMED_16615915}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16615915 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16615915}}
==About this Structure==
==About this Structure==
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[[Category: Template g]]
[[Category: Template g]]
[[Category: Y-family]]
[[Category: Y-family]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:02:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:55:41 2008''

Revision as of 14:55, 27 July 2008

Image:2flp.png

Template:STRUCTURE 2flp

Binary complex of the catalytic core of human DNA polymerase iota with DNA (template G)

Template:ABSTRACT PUBMED 16615915

About this Structure

2FLP is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

An incoming nucleotide imposes an anti to syn conformational change on the templating purine in the human DNA polymerase-iota active site., Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK, Structure. 2006 Apr;14(4):749-55. PMID:16615915

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