2j4m

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{{Seed}}
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{{STRUCTURE_2j4m| PDB=2j4m | SCENE= }}
{{STRUCTURE_2j4m| PDB=2j4m | SCENE= }}
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'''DOUBLE DOCKERIN FROM PIROMYCES EQUI CEL45A'''
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===DOUBLE DOCKERIN FROM PIROMYCES EQUI CEL45A===
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==Overview==
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The assembly into supramolecular complexes of proteins having complementary activities is central to cellular function. One such complex of considerable biological and industrial significance is the plant cell wall-degrading apparatus of anaerobic microorganisms, termed the cellulosome. A central feature of bacterial cellulosomes is a large non-catalytic protein, the scaffoldin, which contains multiple cohesin domains. An array of digestive enzymes is incorporated into the cellulosome through the interaction of the dockerin domains, present in the catalytic subunits, with the cohesin domains that are present in the scaffoldin. By contrast, in anaerobic fungi, such as Piromyces equi, the dockerins of cellulosomal enzymes are often present in tandem copies; however, the identity of the cognate cohesin domains in these organisms is unclear, hindering further biotechnological development of the fungal cellulosome. Here, we characterise the solution structure and function of a double-dockerin construct from the P. equi endoglucanase Cel45A. We show that the two domains are connected by a flexible linker that is short enough to keep the binding sites of the two domains on adjacent surfaces, and allows the double-dockerin construct to bind more tightly to cellulosomes than a single domain and with greater coverage. The double dockerin binds to the GH3 beta-glucosidase component of the fungal cellulosome, which is thereby identified as a potential scaffoldin.
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{{ABSTRACT_PUBMED_17869267}}
==About this Structure==
==About this Structure==
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2J4M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Piromyces_equi Piromyces equi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J4M OCA].
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2J4M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Piromyces_equi Piromyces equi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J4M OCA].
==Reference==
==Reference==
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[[Category: Protein binding]]
[[Category: Protein binding]]
[[Category: Small cysteine-rich]]
[[Category: Small cysteine-rich]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:19:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:01:06 2008''

Revision as of 15:01, 27 July 2008

Image:2j4m.png

Template:STRUCTURE 2j4m

DOUBLE DOCKERIN FROM PIROMYCES EQUI CEL45A

Template:ABSTRACT PUBMED 17869267

About this Structure

2J4M is a Single protein structure of sequence from Piromyces equi. Full experimental information is available from OCA.

Reference

Characterization of a double dockerin from the cellulosome of the anaerobic fungus Piromyces equi., Nagy T, Tunnicliffe RB, Higgins LD, Walters C, Gilbert HJ, Williamson MP, J Mol Biol. 2007 Oct 26;373(3):612-22. Epub 2007 Aug 19. PMID:17869267

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