1yry

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[[Image:1yry.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1yry| PDB=1yry | SCENE= }}
{{STRUCTURE_1yry| PDB=1yry | SCENE= }}
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'''Crystal structure of human PNP complexed with MESG'''
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===Crystal structure of human PNP complexed with MESG===
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==Overview==
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Purine nucleoside phosphorylase (PNP) catalyzes the reversible phosphorolysis of nucleosides and deoxynucleosides, generating ribose 1-phosphate and the purine base, which is an important step of purine catabolism pathway. The lack of such an activity in humans, owing to a genetic disorder, causes T-cell impairment, and drugs that inhibit this enzyme may have the potential of being utilized as modulators of the immunological system to treat leukemia, autoimmune diseases, and rejection in organ transplantation. Here, we describe kinetics and crystal structure of human PNP in complex with 7-methyl-6-thio-guanosine, a synthetic substrate, which is largely used in activity assays. Analysis of the structure identifies different protein conformational changes upon ligand binding, and comparison of kinetic and structural data permits an understanding of the effects of atomic substitution on key positions of the synthetic substrate and their consequences to enzyme binding and catalysis. Such knowledge may be helpful in designing new PNP inhibitors.
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The line below this paragraph, {{ABSTRACT_PUBMED_16154528}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16154528 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16154528}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Kinetics and crystal structure of human purine nucleoside phosphorylase in complex with 7-methyl-6-thio-guanosine., Silva RG, Pereira JH, Canduri F, de Azevedo WF Jr, Basso LA, Santos DS, Arch Biochem Biophys. 2005 Oct 1;442(1):49-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16154528 16154528]
Kinetics and crystal structure of human purine nucleoside phosphorylase in complex with 7-methyl-6-thio-guanosine., Silva RG, Pereira JH, Canduri F, de Azevedo WF Jr, Basso LA, Santos DS, Arch Biochem Biophys. 2005 Oct 1;442(1):49-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16154528 16154528]
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Crystal structure of human purine nucleoside phosphorylase at 2.3A resolution., de Azevedo WF Jr, Canduri F, dos Santos DM, Silva RG, de Oliveira JS, de Carvalho LP, Basso LA, Mendes MA, Palma MS, Santos DS, Biochem Biophys Res Commun. 2003 Aug 29;308(3):545-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12914785 12914785]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Purine-nucleoside phosphorylase]]
[[Category: Purine-nucleoside phosphorylase]]
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[[Category: Purine nucleoside phosphorylase]]
[[Category: Purine nucleoside phosphorylase]]
[[Category: Synchrotron]]
[[Category: Synchrotron]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:42:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:06:59 2008''

Revision as of 15:07, 27 July 2008

Image:1yry.png

Template:STRUCTURE 1yry

Crystal structure of human PNP complexed with MESG

Template:ABSTRACT PUBMED 16154528

About this Structure

1YRY is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Kinetics and crystal structure of human purine nucleoside phosphorylase in complex with 7-methyl-6-thio-guanosine., Silva RG, Pereira JH, Canduri F, de Azevedo WF Jr, Basso LA, Santos DS, Arch Biochem Biophys. 2005 Oct 1;442(1):49-58. PMID:16154528

Crystal structure of human purine nucleoside phosphorylase at 2.3A resolution., de Azevedo WF Jr, Canduri F, dos Santos DM, Silva RG, de Oliveira JS, de Carvalho LP, Basso LA, Mendes MA, Palma MS, Santos DS, Biochem Biophys Res Commun. 2003 Aug 29;308(3):545-52. PMID:12914785

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