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1k3j

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{{STRUCTURE_1k3j| PDB=1k3j | SCENE= }}
{{STRUCTURE_1k3j| PDB=1k3j | SCENE= }}
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'''Refined NMR Structure of the FHA1 Domain of Yeast Rad53'''
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===Refined NMR Structure of the FHA1 Domain of Yeast Rad53===
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==Overview==
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Rad53, a yeast checkpoint protein involved in regulating the repair of DNA damage, contains two forkhead-associated domains, FHA1 and FHA2. Previous combinatorial library screening has shown that FHA1 strongly selects peptides containing a pTXXD motif. Subsequent location of this motif within the sequence of Rad9, the target protein, coupled with spectroscopic analysis has led to identification of a tight binding sequence that is likely the binding site of FHA1: (188)SLEV(pT)EADATFVQ(200). We present solution structures of FHA1 in complex with this pT-peptide and with another Rad9-derived pT-peptide that has ca 30-fold lower affinity, (148)KKMTFQ(pT)PTDPLE(160). Both complexes showed intermolecular NOEs predominantly between three peptide residues (pT, +1, and +2 residues) and five FHA1 residues (S82, R83, S85, T106, and N107). Furthermore, the following interactions were implicated on the basis of chemical shift perturbations and structural analysis: the phosphate group of the pT residue with the side-chain amide group of N86 and the guanidino group of R70, and the carboxylate group of Asp (at the +3 position) with the guanidino group of R83. The generated structures revealed a similar binding mode adopted by these two peptides, suggesting that pT and the +3 residue Asp are the major contributors to binding affinity and specificity, while +1 and +2 residues could provide additional fine-tuning. It was also shown that FHA1 does not bind to the corresponding pS-peptides or a related pY-peptide. We suggest that differentiation between pT and pS-peptides by FHA1 can be attributed to hydrophobic interactions between the methyl group of the pT residue and the aliphatic protons of R83, S85, and T106 from FHA1.
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{{ABSTRACT_PUBMED_11846567}}
==About this Structure==
==About this Structure==
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1K3J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3J OCA].
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1K3J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3J OCA].
==Reference==
==Reference==
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[[Category: Rad53]]
[[Category: Rad53]]
[[Category: Rad9]]
[[Category: Rad9]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:12:30 2008''

Revision as of 15:12, 27 July 2008

Image:1k3j.png

Template:STRUCTURE 1k3j

Refined NMR Structure of the FHA1 Domain of Yeast Rad53

Template:ABSTRACT PUBMED 11846567

About this Structure

1K3J is a Single protein structure of sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA.

Reference

Solution structures of two FHA1-phosphothreonine peptide complexes provide insight into the structural basis of the ligand specificity of FHA1 from yeast Rad53., Yuan C, Yongkiettrakul S, Byeon IJ, Zhou S, Tsai MD, J Mol Biol. 2001 Nov 30;314(3):563-75. PMID:11846567

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