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1nqp

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{{STRUCTURE_1nqp| PDB=1nqp | SCENE= }}
{{STRUCTURE_1nqp| PDB=1nqp | SCENE= }}
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'''Crystal structure of Human hemoglobin E at 1.73 A resolution'''
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===Crystal structure of Human hemoglobin E at 1.73 A resolution===
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==Overview==
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Hemoglobin A(2) (alpha(2)delta(2)), a minor (2-3%) component of circulating red blood cells, acts as an anti-sickling agent and its elevated concentration in beta-thalassemia is a useful clinical diagnostic. In beta-thalassemia major, where there is a failure of beta-chain production, HbA(2) acts as the predominant oxygen delivery mechanism. Hemoglobin E, is another common abnormal hemoglobin, caused by splice site mutation in exon 1 of beta globin gene, when combines with beta-thalassemia, causes severe microcytic anemia. The purification, crystallization, and preliminary structural studies of HbA(2) and HbE are reported here. HbA(2) and HbE are purified by cation exchange column chromatography in presence of KCN from the blood samples of individuals suffering from beta-thalassemia minor and E beta-thalassemia. X-ray diffraction data of HbA(2) and HbE were collected upto 2.1 and 1.73 A, respectively. HbA(2) crystallized in space group P2(1) with unit cell parameters a=54.33 A, b=83.73 A, c=62.87 A, and beta=99.80 degrees whereas HbE crystallized in space group P2(1)2(1)2(1) with unit cell parameters a=60.89 A, b=95.81 A, and c=99.08 A. Asymmetric unit in each case contains one Hb tetramer in R(2) state.
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(as it appears on PubMed at http://www.pubmed.gov), where 12659864 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12659864}}
==About this Structure==
==About this Structure==
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[[Category: Sen, U.]]
[[Category: Sen, U.]]
[[Category: Hemoglobin e oxygen transport beta thalassemia]]
[[Category: Hemoglobin e oxygen transport beta thalassemia]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:52:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:14:30 2008''

Revision as of 15:14, 27 July 2008

Image:1nqp.png

Template:STRUCTURE 1nqp

Crystal structure of Human hemoglobin E at 1.73 A resolution

Template:ABSTRACT PUBMED 12659864

About this Structure

1NQP is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystallization and preliminary X-ray structural studies of hemoglobin A2 and hemoglobin E, isolated from the blood samples of beta-thalassemic patients., Dasgupta J, Sen U, Choudhury D, Datta P, Chakrabarti A, Chakrabarty SB, Chakrabarty A, Dattagupta JK, Biochem Biophys Res Commun. 2003 Apr 4;303(2):619-23. PMID:12659864

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