1jxg

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{{STRUCTURE_1jxg| PDB=1jxg | SCENE= }}
{{STRUCTURE_1jxg| PDB=1jxg | SCENE= }}
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'''The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge'''
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===The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge===
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==Overview==
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Plastocyanin is an electron-transfer protein which has been largely used for biophysical studies as well as for protein-engineering experiments. A surface disulfide bridge has been engineered in poplar plastocyanin to allow protein chemisorption on gold substrates. The mutated plastocyanin crystal structure has been studied at 1.6 A resolution (R factor = 0.145, R(free) = 0.205) to characterize the effects of the engineered disulfide on the overall protein structure and on the Cu-coordination sphere in view of biophysical applications. The new orthorhombic crystal form isolated for the mutated plastocyanin displays two protein molecules per asymmetric unit.
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(as it appears on PubMed at http://www.pubmed.gov), where 11679761 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11679761}}
==About this Structure==
==About this Structure==
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[[Category: Verbeet, M P.]]
[[Category: Verbeet, M P.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:02:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:22:33 2008''

Revision as of 15:22, 27 July 2008

Image:1jxg.png

Template:STRUCTURE 1jxg

The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge

Template:ABSTRACT PUBMED 11679761

About this Structure

1JXG is a Single protein structure of sequence from Populus nigra. Full crystallographic information is available from OCA.

Reference

The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge., Milani M, Andolfi L, Cannistraro S, Verbeet MP, Bolognesi M, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1735-8. Epub 2001, Oct 25. PMID:11679761

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