2h8e

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{{STRUCTURE_2h8e| PDB=2h8e | SCENE= }}
{{STRUCTURE_2h8e| PDB=2h8e | SCENE= }}
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'''Structure RusA D70N'''
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===Structure RusA D70N===
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==Overview==
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We have determined the structure of a catalytically inactive D70N variant of the Escherichia coli RusA resolvase bound to a duplex DNA substrate that reveals critical protein-DNA interactions and permits a much clearer understanding of the interaction of the enzyme with a Holliday junction (HJ). The RusA enzyme cleaves HJs, the fourway DNA branchpoints formed by homologous recombination, by introducing symmetrical cuts in the phosphodiester backbone in a Mg2+ dependent reaction. Although, RusA shows a high level of selectivity for DNA junctions, preferring to bind fourway junctions over other substrates in vitro, it has also been shown to have appreciable affinity for duplex DNA. However, RusA does not show DNA cleavage activity with duplex substrates. Our structure suggests the possible basis for structural selectivity as well as sources of the sequence specificity observed for DNA cleavage by RusA.
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{{ABSTRACT_PUBMED_17028102}}
==About this Structure==
==About this Structure==
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[[Category: Homologous recombination]]
[[Category: Homologous recombination]]
[[Category: Resolvase]]
[[Category: Resolvase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:26:35 2008''

Revision as of 15:26, 27 July 2008

Image:2h8e.png

Template:STRUCTURE 2h8e

Structure RusA D70N

Template:ABSTRACT PUBMED 17028102

About this Structure

2H8E is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

RusA Holliday junction resolvase: DNA complex structure--insights into selectivity and specificity., Macmaster R, Sedelnikova S, Baker PJ, Bolt EL, Lloyd RG, Rafferty JB, Nucleic Acids Res. 2006;34(19):5577-84. Epub 2006 Oct 5. PMID:17028102

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