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2qqu

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{{STRUCTURE_2qqu| PDB=2qqu | SCENE= }}
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'''Crystal structure of a cell-wall invertase (D239A) from Arabidopsis thaliana in complex with sucrose'''
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===Crystal structure of a cell-wall invertase (D239A) from Arabidopsis thaliana in complex with sucrose===
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==Overview==
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In plants, cell-wall invertases fulfil important roles in carbohydrate partitioning, growth, development and crop yield. In this study, we report on different X-ray crystal structures of Arabidopsis thaliana cell-wall invertase 1 (AtcwINV1) mutants with sucrose. These structures reveal a detailed view of sucrose binding in the active site of the wild-type AtcwINV1. Compared to related enzyme-sucrose complexes, important differences in the orientation of the glucose subunit could be observed. The structure of the E203Q AtcwINV1 mutant showed a complete new binding modus, whereas the D23A, E203A and D239A structures most likely represent the productive binding modus. Together with a hydrophobic zone formed by the conserved W20, W47 and W82, the residues N22, D23, R148, E203, D149 and D239 are necessary to create the ideal sucrose-binding pocket. D239 can interact directly with the glucose moiety of sucrose, whereas K242 has an indirect role in substrate stabilization. Most probably, K242 keeps D239 in a favourable position upon substrate binding. Unravelling the exact position of sucrose in plant cell-wall invertases is a necessary step towards the rational design of superior invertases to further increase crop yield and biomass production.
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{{ABSTRACT_PUBMED_18258263}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Invertase]]
[[Category: Invertase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:41:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:37:33 2008''

Revision as of 15:37, 27 July 2008

Image:2qqu.png

Template:STRUCTURE 2qqu

Crystal structure of a cell-wall invertase (D239A) from Arabidopsis thaliana in complex with sucrose

Template:ABSTRACT PUBMED 18258263

About this Structure

2QQU is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.

Reference

Crystal structures of Arabidopsis thaliana cell-wall invertase mutants in complex with sucrose., Lammens W, Le Roy K, Van Laere A, Rabijns A, Van den Ende W, J Mol Biol. 2008 Mar 21;377(2):378-85. Epub 2008 Jan 5. PMID:18258263

Page seeded by OCA on Sun Jul 27 18:37:33 2008

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