1pio

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{{STRUCTURE_1pio| PDB=1pio | SCENE= }}
{{STRUCTURE_1pio| PDB=1pio | SCENE= }}
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'''AN ENGINEERED STAPHYLOCOCCUS AUREUS PC1 BETA-LACTAMASE THAT HYDROLYSES THIRD GENERATION CEPHALOSPORINS'''
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===AN ENGINEERED STAPHYLOCOCCUS AUREUS PC1 BETA-LACTAMASE THAT HYDROLYSES THIRD GENERATION CEPHALOSPORINS===
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==Overview==
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The beta-lactamase from Staphylococcus aureus PC1 has been cloned into an Escherichia coli vector for site-directed mutagenesis and high-level protein expression. A mutant enzyme has been produced in which Ala238 is replaced by a serine, and Ile239 is deleted (A238S:I239del). The engineered enzyme hydrolyses third-generation cephalosporins substantially more rapidly than the parental enzyme does, while hydrolysis of benzylpenicillin is slower with the mutant than with the wild-type and native enzymes. The mutant beta-lactamase has been crystallized and the structure determined and refined at 2.8 A resolution. The disposition of the beta-strand which forms the side of the active site is altered in comparison with the native S. aureus beta-lactamase structure, widening the active site cleft and providing space to accommodate the bulky side-chains of the third-generation cephalosporins.
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(as it appears on PubMed at http://www.pubmed.gov), where 8869640 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8869640}}
==About this Structure==
==About this Structure==
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[[Category: Herzberg, O.]]
[[Category: Herzberg, O.]]
[[Category: Zawadzke, L E.]]
[[Category: Zawadzke, L E.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:07:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:55:19 2008''

Revision as of 15:55, 27 July 2008

Image:1pio.png

Template:STRUCTURE 1pio

AN ENGINEERED STAPHYLOCOCCUS AUREUS PC1 BETA-LACTAMASE THAT HYDROLYSES THIRD GENERATION CEPHALOSPORINS

Template:ABSTRACT PUBMED 8869640

About this Structure

1PIO is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

An engineered Staphylococcus aureus PC1 beta-lactamase that hydrolyses third-generation cephalosporins., Zawadzke LE, Smith TJ, Herzberg O, Protein Eng. 1995 Dec;8(12):1275-85. PMID:8869640

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