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2e0j

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[[Image:2e0j.jpg|left|200px]]
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{{STRUCTURE_2e0j| PDB=2e0j | SCENE= }}
{{STRUCTURE_2e0j| PDB=2e0j | SCENE= }}
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'''Mutant Human Ribonuclease 1 (R31L, R32L)'''
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===Mutant Human Ribonuclease 1 (R31L, R32L)===
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==Overview==
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A protein crystal lattice consists of surface contact regions, where the interactions of specific groups play a key role in stabilizing the regular arrangement of the protein molecules. In an attempt to control protein incorporation in a crystal lattice, a leucine zipper-like hydrophobic interface (comprising four leucine residues) was introduced into a helical region (helix 2) of the human pancreatic ribonuclease 1 (RNase 1) that was predicted to form a suitable crystallization interface. Although crystallization of wild-type RNase 1 has not yet been reported, the RNase 1 mutant having four leucines (4L-RNase 1) was successfully crystallized under several different conditions. The crystal structures were subsequently determined by X-ray crystallography by molecular replacement using the structure of bovine RNase A. The overall structure of 4L-RNase 1 is quite similar to that of the bovine RNase A, and the introduced leucine residues formed the designed crystal interface. To characterize the role of the introduced leucine residues in crystallization of RNase 1 further, the number of leucines was reduced to three or two (3L- and 2L-RNase 1, respectively). Both mutants crystallized and a similar hydrophobic interface as in 4L-RNase 1 was observed. A related approach to engineer crystal contacts at helix 3 of RNase 1 (N4L-RNase 1) was also evaluated. N4L-RNase 1 also successfully crystallized and formed the expected hydrophobic packing interface. These results suggest that appropriate introduction of a leucine zipper-like hydrophobic interface can promote intermolecular symmetry for more efficient protein crystallization in crystal lattice engineering efforts.
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{{ABSTRACT_PUBMED_17586772}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Mutant human pancreatic ribonuclease 1]]
[[Category: Mutant human pancreatic ribonuclease 1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 01:42:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:57:17 2008''

Revision as of 15:57, 27 July 2008

Image:2e0j.png

Template:STRUCTURE 2e0j

Mutant Human Ribonuclease 1 (R31L, R32L)

Template:ABSTRACT PUBMED 17586772

About this Structure

2E0J is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

'Crystal lattice engineering,' an approach to engineer protein crystal contacts by creating intermolecular symmetry: crystallization and structure determination of a mutant human RNase 1 with a hydrophobic interface of leucines., Yamada H, Tamada T, Kosaka M, Miyata K, Fujiki S, Tano M, Moriya M, Yamanishi M, Honjo E, Tada H, Ino T, Yamaguchi H, Futami J, Seno M, Nomoto T, Hirata T, Yoshimura M, Kuroki R, Protein Sci. 2007 Jul;16(7):1389-97. PMID:17586772

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