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| | {{STRUCTURE_1shn| PDB=1shn | SCENE= }} | | {{STRUCTURE_1shn| PDB=1shn | SCENE= }} |
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| - | '''Crystal structure of shrimp alkaline phosphatase with phosphate bound'''
| + | ===Crystal structure of shrimp alkaline phosphatase with phosphate bound=== |
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| - | ==Overview==
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| - | Alkaline phosphatases (APs) are homodimeric metalloenzymes that catalyze the hydrolysis and transphosphorylation of phosphate monoesters. Each monomer contains a metal-binding triad that for optimal activity is usually occupied by two zinc ions and one magnesium ion. The recently determined crystal structure of cold-active shrimp alkaline phosphatase (SAP) was, however, fully occupied by zinc ions. This paper describes a metal-exchange experiment in which the zinc ion in one binding site (referred to as the M3 site) is replaced by magnesium. Crystal structures revealed a concomitant structural change: the metal exchange causes movement of a ligating histidine into a conformation in which it does not coordinate to the metal ion. The M3 site is relevant to catalysis: its occupation by magnesium is postulated to favour catalysis and it has been suggested to be a regulatory site for other APs. Further crystallographic studies show that ligand binding can induce a conformational change of an active-site arginine from a 'non-docked' (non-interacting) to a 'docked' conformation (interacting with the ligand). The first conformation has only been observed in SAP, while the latter is common in available AP structures. The observation that the arginine does not always bind the substrate may explain the increased catalytic efficiency that is generally observed for cold-active enzymes.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_15333925}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15333925 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15333925}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Zinc]] | | [[Category: Zinc]] |
| | [[Category: Zinc triad]] | | [[Category: Zinc triad]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:42:54 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:59:08 2008'' |
Revision as of 15:59, 27 July 2008
Template:STRUCTURE 1shn
Crystal structure of shrimp alkaline phosphatase with phosphate bound
Template:ABSTRACT PUBMED 15333925
About this Structure
1SHN is a Single protein structure of sequence from Pandalus borealis. Full crystallographic information is available from OCA.
Reference
Ligand-binding and metal-exchange crystallographic studies on shrimp alkaline phosphatase., de Backer MM, McSweeney S, Lindley PF, Hough E, Acta Crystallogr D Biol Crystallogr. 2004 Sep;60(Pt 9):1555-61. Epub 2004, Aug 26. PMID:15333925
Page seeded by OCA on Sun Jul 27 18:59:08 2008
