2zhc

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{{Seed}}
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{{STRUCTURE_2zhc| PDB=2zhc | SCENE= }}
{{STRUCTURE_2zhc| PDB=2zhc | SCENE= }}
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'''ParM filament'''
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===ParM filament===
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==Overview==
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ParM is a prokaryotic actin homologue, which ensures even plasmid segregation before bacterial cell division. In vivo, ParM forms a labile filament bundle that is reminiscent of the more complex spindle formed by microtubules partitioning chromosomes in eukaryotic cells. However, little is known about the underlying structural mechanism of DNA segregation by ParM filaments and the accompanying dynamic instability. Our biochemical, TIRF microscopy and high-pressure SAX observations indicate that polymerization and disintegration of ParM filaments is driven by GTP rather than ATP and that ParM acts as a GTP-driven molecular switch similar to a G protein. Image analysis of electron micrographs reveals that the ParM filament is a left-handed helix, opposed to the right-handed actin polymer. Nevertheless, the intersubunit contacts are similar to those of actin. Our atomic model of the ParM-GMPPNP filament, which also fits well to X-ray fibre diffraction patterns from oriented gels, can explain why after nucleotide release, large conformational changes of the protomer lead to a breakage of intra- and interstrand interactions, and thus to the observed disintegration of the ParM filament after DNA segregation.
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(as it appears on PubMed at http://www.pubmed.gov), where 18188150 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18188150}}
==About this Structure==
==About this Structure==
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[[Category: Plasmid]]
[[Category: Plasmid]]
[[Category: Plasmid partition]]
[[Category: Plasmid partition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:12:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:03:38 2008''

Revision as of 16:03, 27 July 2008

Image:2zhc.png

Template:STRUCTURE 2zhc

ParM filament

Template:ABSTRACT PUBMED 18188150

About this Structure

2ZHC is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Molecular structure of the ParM polymer and the mechanism leading to its nucleotide-driven dynamic instability., Popp D, Narita A, Oda T, Fujisawa T, Matsuo H, Nitanai Y, Iwasa M, Maeda K, Onishi H, Maeda Y, EMBO J. 2008 Feb 6;27(3):570-9. Epub 2008 Jan 10. PMID:18188150

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